Methods for inhibiting bacterial cytotoxicity

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Having -c- – wherein x is chalcogen – bonded directly to...

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514 6, 514 34, A61K 3135, A61K 3816, A61K 3170

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active

059488158

ABSTRACT:
The invention provides methods and compositions for reducing microbial cytotoxicity to a cell, by contacting a cell subject to extracellular microbial cytotoxicity with an effective amount of at least one of genistein or a genistein derivative. In preferred embodiments, the microbe is a gram-negative bacterium, a non-Enterobacteriaceae or a Pseudomonas aeruginosa or cepacia and the host cell is a mammalian epithelial cell, especially a lung epithelial cell or a corneal epithelial cell. The genistein or genistein derivative may be administered prophylactically prior to infection, subsequent thereto and/or in conjunction with conventional antibiotic therapies.

REFERENCES:
Sandros et al. Cellular Events concurrent with Porphyromonas Gingivalis Invasion of Oral Epithelium In Vitro. Europ. J. Oral Sci. 1996 vol. 104, pp. 363-371, see abst.
Ralph et al. Resistance of Melanoma Cell Lines to Interferons Correlates with Reduction of IFN-Induced Tyrosine Phosphorylation: Induction of the Anti-Viral State by IFN is Prevented by Tyrosine Kinase Inhibitors. J. Immunol. 1995, vol. 154, No. 5, pp. 2248-2256, see abst.
Valentin-Weigand et al. Characterization of Group B Streptococcal Invasion in HEP-2 Epithelial Cells. FEMS Microbiol. Letters. Feb. 1997. Vo. 147, pp. 69-75, esp. p. 70, 2.sup.nd col.
Bandyukova et al., Chemical Abstract 108:71937 (1987) copy of abstract.
Windholz et al. The Merck Index (1983) abstracts Nos. 937, 4251, and 9318.

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