Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-12-13
2005-12-13
Nguyen, Dave Trong (Department: 1632)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S094000, C435S325000, C435S455000, C536S023100, C536S023500, C536S024100, C514S04400A, C424S093100, C800S021000, C204S450000
Reexamination Certificate
active
06974671
ABSTRACT:
In accordance with the present invention, it has been discovered that CREB regulates hepatic gluconeogenesis via the co-activator, PGC-1. PGC-1 potentiated glucocorticoid induction of the gene for PEPCK, the rate limiting enzyme in gluconeogenesis, via the glucocorticoid response unit in the promoter, indicating that activation of PGC-1 by CREB in liver contributes to the pathogenesis of diabetes mellitus. In accordance with the above discoveries, the present invention provides a method of identifying a compound that modulates gluconeogenesis. The invention method comprises contacting CREB and a nucleic acid comprising a PGC-1 promoter with a test compound, and determining if the test compound modulates binding between CREB and the PGC-1 promoter.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5082830 (1992-01-01), Brakel et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5242974 (1993-09-01), Holmes
patent: 5288514 (1994-02-01), Ellman
patent: 5312233 (1994-05-01), Tanny et al.
patent: 5384261 (1995-01-01), Winkler et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5436327 (1995-07-01), Southern et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5472672 (1995-12-01), Brennan
patent: 5527681 (1996-06-01), Holmes
patent: 5529756 (1996-06-01), Brennan
patent: 5532128 (1996-07-01), Eggers et al.
patent: 5545531 (1996-08-01), Rava et al.
patent: 5554501 (1996-09-01), Coassin et al.
patent: 5556752 (1996-09-01), Lockhart et al.
patent: 5561071 (1996-10-01), Hollenberg et al.
patent: 5571639 (1996-11-01), Hubbell et al.
patent: 5593839 (1997-01-01), Hubbell et al.
patent: 5599695 (1997-02-01), Pease et al.
patent: 5624711 (1997-04-01), Sundberg et al.
patent: 5658734 (1997-08-01), Brock et al.
patent: 5677195 (1997-10-01), Winkler et al.
patent: 5700637 (1997-12-01), Southern
patent: 5945526 (1999-08-01), Lee et al.
patent: 6004755 (1999-12-01), Wang
patent: 6017696 (2000-01-01), Heller
patent: 6024138 (2000-02-01), Fritz et al.
patent: 6051380 (2000-04-01), Sosnowski et al.
patent: 6110426 (2000-08-01), Shalon et al.
patent: 6129828 (2000-10-01), Sheldon, III et al.
patent: WO 97/27317 (1997-07-01), None
Vidal-Puig, et al. (2001) Nature, 413: 125-26.
Yoon, et al. (2001) Nature, 413: 131-38.
Herzig, et al. (2001) Nature, 413: 179-83.
Herzig, et al. (2001) Nature, 413: 652.
Deonarain (1998) Exp. Opin. Ther. Pat., 8(1): 53-69.
Gorecki (2001) Exp. Opin. Emerging Drugs, 6(2): 187-98.
Verma, et al. (1997) Nature, 389: 239-42.
Eck, et al. (1996) Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th Ed., McGraw-Hill, New York, NY., pp. 77-101.
Ahn et al., “A dominant-negative inhibitor of CREB reveals that it is a general mediator stimulus-dependent transcription ofc-fos.” Molec. Cell. Biol., 18(2): 967-977 (1998).
Becker et al., “Use of recombinant adenovirus for metabolic engineering of mammalian cells.” Methods Cell Biol, 43:161-189 (1994).
Brown & Botstein, “Exploring the new world of the genome with DNA microarrays,” Nature Genetics Supp., 21:33-37, 1999.
Chalifour et al., “A method for analysis of gene expression patterns,” Anal. Biochem, 216: 299-304, 1994.
Chen et al., “Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene indb/dbmice.” Cell, 84:491-495, 1996.
Cheung et al, “Making and reading microarrays,” Nature Genetics Supp., 21:15-19, 1999.
Consoli, A., “Role of liver in pathophysiology of NIDDM.” Diabetes Care, 15:430-441, 1992.
Cook et al., “Segregation analysis of NIDDM in Caucasian families.” Diabetologia, 37:1231-1240, 1994.
DeFronzo, R.A., “The Triumvirate: β-Cell, muscle, liver, a collusion responsible for NIDDM.” Diabetes, 37:667-687, 1988.
Fire et al., “Potent and specific genetic interference by double-stranded RNA inCaenorhabditis elegans.” Nature, 391:806-811, 1998.
Friedman et al., “Phosphoenolpyruvate carboxykinase (GTP) gene transcription and hyperglycemia are regulated by glucocorticoids in genetically obese db/db transgenic mice.” J. Biol Chem, 272: 31475-31481, 1997.
Gonzalez, G. & Montminy, M., “Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at Serine 133.” Cell, 59:675-680, 1989.
Harris et al., “Onset of NIDDM occurs at least 4-7 Yr before clinical diagnosis.” Diabetes Care, 15:815-819, 1992.
He et al., “A simplified system for generating recombinant adenoviruses.” Proc. Natl. Acad. Sci., 95:2509-2514, 1998.
Hong et al., “Sequencing of large double-stranded DNA using the dideoxy sequencing technique.” Bioscience Reports, 2: 907-912, 1982.
Houghten et al., “Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery.” Nature, 354:84-86, 1991.
Imai et al., “Characterization of a complex glucocorticoid response unit in the phosphoenolpyruvate carboxykinase gene.” Mol Cell Biol, 10: 4712-4719, 1990.
Jitrapakdee et al., “The rat pyruvate carboxylase gene structure: Alternate promoters generate multiple transcripts with the 5′-end heterogeneity.” J Biol Chem, 272: 20522-20530, 1997.
Ju et al., “Design and synthesis of fluorescence energy transfer dye-labeled primers and their application for DNA sequencing and analysis.” Anal. Biochem. 231(1):131-140, 1995.
Ju et al., “Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.” Proc. Nat. Acad. Sci. (USA) 92:4347-4351, 1995.
Kennerdell and Carthew, “Use of dsRNA-mediated genetic interference to demonstrate thatfrizzledandfrizzled2 act in the wingless pathway.” .Cell, 95:1017-1026, 1998.
King et al., “Global socioeconomic development trends (1985-1988).” Wld. Health Statistical Quarterly, 42:190-196, 1989.
Knutti et al., “A tissue-specific coactivator of steroid receptors, identified in a functional genetic screen.” Mol Cell Biol , 20:2411-2422, 2000.
Kobberling, J., “Studies on the genetic heterogeneity of diabetes mellitus.” Diabetologia 7:46-49, 1971.
Lam et al., “A new type of synthetic peptide library for identifying ligand-binding activity.” Nature, 354:82-84, 1991.
Lee et al., “Abnormal splicing of the leptin receptor indiabeticmice,” Nature, 379: 632-635, 1996.
Lei et al., “Glucose-6-phosphatase dependent substrate transport in the glycogen storage disease type-1a mouse.” Nat Genet, 13: 203-209, 1996.
Lipshutz et al., “High density synthetic oligonucleotide arrays.” Nature Genetics Supp., 21:20-24, 1999.
Liu et al., “The mouse albumin promoter and a distal upstream site are simultaneously DNase I hypersensitive in liver chromatin and bind similar liver-abudant factors in vitro.” Genes Dev, 2: 528-541, 1988.
Liu et al., “Cyclic AMP induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription is mediated by multiple promoter elements.” J Biol Chem, 266:19095-19102, 1991.
Long et al., “The CREB family of activators is required for endochondral bone development.” Development 128:541-550, 2001.
McGraw, R., “Dideoxy DNA sequencing with end-labeled oligonucleotide primers,” Anal. Biochem., 143: 298-303, 1984.
Michael et al., “The phosphorylation status of a cyclic AMP-responsive activator is modulated via a chromatin-dependent mechanism.” Mol Cell Biol., 20:1596-1603, 2000.
Nakajima et al., “RNA Helicase A mediates association of CBP with RNA polymerase II.” Cell, 90:1107-1112, 1997.
Newman et al., “Concordance for Type 2 (non-insulin-dependent) diabetes mellitus in male twins.&#
Herzig Stephan
Montminy Marc R.
Spiegelman Bruce M.
Foley & Lardner
Kelly Robert M.
Nguyen Dave Trong
Reiter Stephen E.
Salk Institute for Biological Studies
LandOfFree
Methods for indentifying compounds that modulate... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for indentifying compounds that modulate..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for indentifying compounds that modulate... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3520986