Methods for inactivating nucleotide sequences and metal chelates

Details Methods for inactivating nucleotide sequences and metal chelates Methods for inactivating nucleotide sequences and metal chelates

1992-04-03

1999-01-12

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435810, 536 231, 536 281, 546 1, 546 4, 546 10, 546 26, 546 88, 935 77, 935 78, C12Q 168, C12P 1934, C07H 204, C07D22102

Patent

active

058586504

ABSTRACT:
This invention relates to methods, reagents, and kits utilizing metal chelates to inactivate nucleotide sequences, especially to inactivate polymerase chain reaction (PCR) and ligase chain reaction (LCR) products and to inactivate nucleotide sequences in a bioprocess and bioproduct. A novel metal chelate class is also disclosed.

REFERENCES:
Karn et al. Nature (1966) 211: 160-162.
Thederahn et al J. Am Chem Soc (1989) 111: 4941-4945.
Yamamoto, K. J. Biol Chem (1989) 264: 15435-15440.
Furrer et al. Nature (1990) 346: 324.

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