Methods for in vitro protein synthesis

Details Methods for in vitro protein synthesis Methods for in vitro protein synthesis

1997-06-05

1999-10-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4353171, 435820, C12P 2100, C12N 100, C07K 1400

Patent

active

059687673

ABSTRACT:
A method is provided for in vitro protein synthesis in a bacterial extract wherein the reaction mixture comprises a reducing agent and dissolved oxygen (DO.sub.2) wherein the DO.sub.2 concentration is regulated such that complete oxidation of the reducing agent concentration does not occur for a period of at least about 30 minutes following initiation of protein synthesis in the reaction mixture. Also provided is a method for in vitro protein synthesis in bacterial extracts wherein the reaction mixture comprises an initial methionine concentration of at least about 1.0 mM, or wherein the reaction mixture comprises both labeled and unlabeled methionine, and the initial concentration of unlabeled methionine is at least about 0.1 mM.

REFERENCES:
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