Methods for improving transformation efficiency

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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800288, 8003001, 800320, 8003201, 8003203, 435 691, 435413, 435418, 435419, 435440, 435468, 536 236, 536 237, C12N 504, C12N 1511, C12N 1582, A01H 500, A01H 510

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active

060969474

ABSTRACT:
The present invention is drawn to compositions and methods for improving transformation efficiency. The compositions, synthetic marker genes, are used in transformation methods and result in increased transformation efficiency. The synthetic marker genes can be designed for maximum expression in any system.

REFERENCES:
patent: 5380831 (1995-01-01), Adang et al.
Ye et al, Plant Cell Rep., vol. 15, pp. 479-483, 1996.
Shimada et al, Nucl. Acids Res., vol. 19, pp. 983-995, 1991.
Omirulleh et al, Plant Mol. Biol., vol. 21, pp. 415-428, 1993.
Svab et al, Proc. Natl. Acad. Sci. USA, vol. 90, pp. 913-917, 1993.
Galbraith et al, Meth. Cell Biol., vol. 50, pp. 3-14, 1995.
Ortiz et al., "Hygromycin Resistance as an Efficient Selectable Marker for Wheat Stable Transformation", Plant Cell Reports, 15:877-881 (1996).
Chamberlain et al., "The Use of the Emu Promoter with Antibiotic and Herbicide Resistance Genes for the Selection of Transgenic Wheat Callus and Rice Plants", Aust. J. Plant Physiol., 21:95-112 (1994).
Wohlleben et al., Nucleotide Sequence of the Phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TU494 and Its Expression in Nicotiana tabacum Gene, 70:25-37 (1998).
Perez et al., "Phleomycin Resistance as a Dominant Selectable Marker for Plant Cell Transformation", Plant Molecular Biology, 13:365-373 (1989).
Li et al., "Agronomic Trait Evaluation of Field-Grown Transgenic Rice Plants Containing the Hygromycin Resistance Gene and the Maize Activator Element", Plant Science, 108:219-227 (1995).
David Bradley, "Genetic Weeding and Feeding for Tobacco Plants", New Scientest, Jan. 4, 1992, p. 11.
Chiu et al., "Engineered GFP as a Vital Reporter in Plants", Current Biology, vol. 6, No. 3:325-330 (1996).
Maier-Greiner et al., "Isolation and Properties of a Nitrile Hydratase from the Soil Fungus Myrothecium verrucaria That is Highly Specific for the Fertilizer Cyanamide and Cloning of Its Gene", Proc. Natl. Acad. Sci., 88:4260-4264, (May 1991).
Kain et al., "Green Fluorescent Protein as a Reporter of Gene Expression and Protein Localization", BioTechniques, 19:650-655 (Oct. 1995).
David Bradley, "Genetic Weeding and Feeding for Tobacco Plants", New Scientest, Jan. 4, 1992, p. 11.
Goddijn et al., "A Chimaeric Tryptophan Decarboxylase Gene as a Novel Selectable Marker in Plant Cells", Plant Molecular Biology, 22:907-912 (1993).
Perlak et al., "Modification of the Coding Sequence Enhances Plant Expression of Insect Control Protein Genes", Proc. Natl. Acad. Sci. USA, 88:3324-3328 (Apr. 1991).
Perl et al., "Bacterial Dihydrodipicolinate Synthase and Desensitized Aspartate Kinase: Two Novel Selectable Markers for Plant Transformation", Bio/Technology, 11:715-718 (Jun. 1993).
Sagnard et al., "Selection of Transgenic Flax Plants is Facilitated by Spectinomycin", Transgenic Research, 5:131-137 (1996).
Meijer et al., "Transgenic Rice Cell Lines and Plants: Expression of Transferred Chimeric Genes", Plant Molecular Biology, 16:807-820 (1991).
Toki et al., "Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants", Plant Physiol., 100:1503-1507 (1992).
Fennoy et al., "Synonymous Codon Usage in Zea mays L. Nuclear Genes Is Varied by Levels of C and G-Ending Codons", Nucleic Acids Research, vol. 21, No. 23:5294-5300.
Murray et al., "Codon Usage in Plant Genes", Nucleic Acids Research, 17:477-498.
Vaeck et al., "Transgenic Plants Protected from Insect Attack", Nature 328:33-37 (Jul. 2, 1987).
Gallo-Meagher et al., "Herbicide Resistant Transgenic Sugarcane Plants Containing the bar Gene", Crop Sci., 36:1367-1374 (1996).
Maier-Greiner et al., "Herbicide Resistance in Transgenic Plants through Degradation of the Phytotoxin in Urea" Angew. Chem. Int. Ed. Engl., vol. 30, No. 10:1314-1315 (1991).
D'Halluin et al., "The bar Gene as Selectable and Screenable Marker in Plant Engineering", Gene, 103, 171:415-426, Academic Press, (1992).
Rathorn et al., "Use of Bar as a Selectable Marker Gene and for the Production of Herbicide-Resistant Rice Plants from Protoplasts", Plant Molecular Biology 2:871-884 (1993).
Tian et al., "High Level of Expression ofModified Green Fluorescent Protein Gene Transfer in Conifer Tissues", In Vitro 32:311 (1996).
Chiu et al. "Engineered GFP as a Vital Reporter in Plants", Current Biology 6:325-330 (1996).
Pang et al., "An Improved Green Fluorescent Protein Gene as a Vital Marker in Plants", Plant Physiology 112:893-900 (1996).

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