Methods for improving secondary metabolite production in fungi

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...

Reexamination Certificate

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C435S043000, C435S254110, C435S471000

Reexamination Certificate

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06949356

ABSTRACT:
The invention relates to the production of secondary metabolites by fungi. More particularly, the invention relates to improvement of production of commercially important secondary metabolites by fungi. The invention provides methods for improving secondary metabolite production in a fungus, comprising modulating the expression of a gene involved in regulation of secondary metabolite production.

REFERENCES:
patent: 5665543 (1997-09-01), Foulkes et al.
patent: 357119 (1989-08-01), None
patent: WO 99/25735 (1999-05-01), None
patent: WO 99/25865 (1999-05-01), None
patent: WO 00/37629 (2000-06-01), None
patent: WO 01/42426 (2000-12-01), None
Pfeifer et al. Biosynthesis of polyketides in heterologous hosts. Microbiology and Molecular Biology Reviews: 106-118, Mar. 2001.
Fillinger et al. FEBS Letters 368: 547-550, 1995.
Chang et al., Cloning of theAspergillus parasiticusapa-2 gene associated with the regualtion of aflatoxin biosynthesis; Appl Environ Microbiol 59:3273-3279 (1993).
Kennedy and Turner, alpha-(L-alpha Aminoadipyl)-L-cysteinyl-D-valine synthetase is a rate limiting enzyme for penicillin production inAspergillus nidulans; Mol Gen Genet 253:189-197 (1996).
Theilgaard et al., Quantitative Analysis of Penicillium chrysogenum Wis54-1255 Transformants Overexpressing the Penicillin Biosynthetic Genes; Biotech Bioeng 72:379-388 (2001).
Tag et al., G-protein signalling mediates differential production of toxic secondary metabolites; Mol Microbiol 38:658-665 (2000).
Agathos, S.N. et al., “Physiological and Genetic Factors for Process Development of Cyclosporine Fermentations”, J. Ind. Microbiol. 1:39-48 (1986).
Alarco, Anne-Marie et al., “AP1-mediated Multidrug Resistance inSaccharomyces cerevisiaeRequired FLR1 Encoding a Transporter of the Major Facilitator Superfamily”, J. Biol. Chem. 272: 19304-13 (1997).
Alberts, A.W. et al., “Mevinolin: A Highly Potent Competitive Inhibitor of Hydroxymethylglutaryl-coenzyme A Reductase and a Cholesterol-lowering Agent”, Proc. Nat. Sci. USA 77:3957-3961 (1980).
Alexander, N.J. et al. “TRI12, a Trichothecene Efflux Pump fromFusarium sporotrichioides: Gene Isolation and Expression in Yeast”, Mol. Gen. Genet. 261:977-84 (1999).
Bartolomei, Marisa S. et al., “Genetic Analysis of the Repetitive Carbosyl-Terminal Domain of the Largest Subunit of Mouse RNA Polymerase II”, Mol. Cell. Biol. 8:330-9 (1988).
Beltzer, James P. et al., “Yeast LEU4 Encodes Mitochondrial and Nonmitochondrial Forms of α-Isopropylmalate Synthase”, J. Biol. Chem. 263: 368-74 (1988).
Bentley Ronald, “Secondary Metabolite Biosynthesis: The First Century”, Crt. Rev. Biotechnol. 19:1-40 (1999).
Boret, J.F. edit., “Ciciosporin and its Future”, Prog. Allergy 38:9-18 (1986).
Boyum, Rodney et al., “Effect of ATP Binding Cassette/Multidrug Resistance Proteins on ATP Efflux ofSaccharomyces cerevisiae”, Biochem. Biophys. Res. Commun. 230:22-6 (1997).
Brakhage, Axel A., “Molecular Regulation of β-Lactam Biosynthesis in Filamentous Fungi”, Microbiol. Mol. Biol. R v. 62:547-85 (1998).
Buckland, Barry, et al., “Production of Lovastatin, an Inhibitor of Cholesterol Accumulation in Humans”, Topics in Industrial Microbiology: Novel Microbial Products for Medicine and Agriculture, pp. 161-169, Elsevier, Amsterdam (1989).
Bundgaard, H. et al., “A New Spectrophotometric Method for the Determination of Penicillins”,Journal of Pharm. Pharmac. 24:790-794 (1972).
Casquiero, Javier et al., “Gene Targeting inPenicillium chrysogenum: Disruption of the lys2 Gene Leads to Penicillin Overproduction”, J. Bacteriol. 181:1181-1188 (1999).
Cardenas, Maria et al., “Targets of Immunophilin-immunouppressant Complexes are Distinct Highly Conserved Regions of Calcineurin A”, EMBO J 14: 2772-83 (1995).
Chen, Yan et al., “Mapping Mutations in Genes Encoding the Two Large Subunits ofDrosophilaRNA Polymerase II Defines Domains Essential for Basic Transcription Functions and for Proper Expression of Developmental Genes”, Mol. Cell. Biol. 13:4214-22 (1983)
Decottignies, Anabelle et al., “Complete Inventory of the Yeast ABC Proteins”, Nat Genet. 15:137-45 (1997).
Demain, Arnold L., Microbial Secondary Metabolism: A New Theoretical Frontier for Academia, a New Opportunity for Industry:, Ciba Found Symp. 171:3-16 (1992).
Donald, K. Allen et al., “Effects of Overproduction of the Catalytic Domain of 3-Hydroxy-3-Methylgutaryl Coenzyme A Reductase on Squalene Synthesis inSaccharomyces cerevisiae”, Appl. Environ. Microbiol. 63:3341-4 (1997).
Douglas, C.M. et al., “Identification of the FKS1 GeneCandida albicansas the Essential Target of 1,3-62-D-Glucan Synthase Inhibitors”, Antimicrob. Agents Chemother. 41:2471-9 (1997).
Drgonova, Jana et al., “Rho1p, a Yeast Protein at the Interface Between Cell Polarization and Morphogenesis”, Science 272:277-279 (1996).
Feller, Andre et al., “InSaccharomyces cerevisae, Feedback Inhibition of Homocitrate Synthase Isoenzymes by Lysine Modulates the Activation of LYS Gene Expression by Lys14p”, Eur. J. Biochem. 261: 163-70 (1999).
Gonzalez-Garay, Manuel L. et al., “A β-Tubulin Leucine Cluster Involved in Microtubule Assembly and Paclitaxel Resistance”, J. Biol. Chem. 274:23875-82 (1999).
Griffin, DH, ed. “Secondary (Special) Metabolism” Fungal Physiology, Ch. 9 pp. 246-274 ed. (1994).
Heinisch, Jurgen, J. et al., “The Protein Kinase C-mediated MAP Kinase Pathway Involved in the Maintenance of Cellular Integrity inSaccharomyces cerevisiae”, Mol. Microbiol. 32:671-680 (1999).
Jensen, S.E. et al., “Beta-Lactams” Biotechnology 28:239-68 (1995).
Katzmann, David J. et al., “Transcriptional Control of the Yeast PDR5 Gene by the PDR3 Gene Product”, Mol. Cell. Biol. 14:4653-61 (1994).
Kennedy, Jonathan et al., “Modulation of Polyketide Synthase Activity by Accessory Proteins During Lovastatin Biosynthesis”, Science 284:1368-72 (1999).
Lowther, W. Todd et al., “The Anti-angiogenic Agent Fumagillin Covalently Modifies a Conserved Active-site Histidine in theEscherichia coliMethionine Aminopeptidase”, Proc. Natl. Sci. USA 95:12153-7 (1998).
Luengo, Jose et al., “Penicillin Biosynthesis”, Prog. Ind. Microbiol 29:603-38 (1994).
Lum, Pek Yee et al., “Molecular, Functional and Evolutionary Characterization of the Gene Encoding HMG-CoA Reductase in the Fission Yeast,Schizosaccharomyces prombe”, Yeast 12:1107-24 (1996).
Mingot, Jose et al., “Disruption of phacA, anAspergillus nidulansGene Encoding a Novel Cytochrome P450 Monoxygenase Catalyzing Phenylacetate 2-Hydroxylation, Results in Penicillin Overproduction”, J. Biol. Chem. 21:14545-14550 (1999).
Nakaune, Ryoji et al., “A Novel ATP-Binding Cassette Transporter Involved in Multidrug Resistance in the Phytopathogenic FungusPenicillium digitatum”, Appl. Environ. Microbiol. 64:3983-8 (1998).
Nelisssen, Bart et al., “Classification of All Putative Permeases and Other Membrane Plurispanners of the Major Facilitator Superfamily Encoded by the Complete Genome ofSaccharomyces cervisiae”, FEMS Microbiol. Rev. 21:113-34 (1997).
Nonaka, Hidetaro et al., “a Downstream Target of RHO1 Small GTP-binding Protein is PKC1, a Homolog of Protein Kinase C., Which Leads to Activation of the MAP Kinase Cascade inSaccharomyces cerevisiae”, EMBO J. 14:5931-5938 (1995).
Norman, Thea C. et al., “Genetic Selection of Peptide Inhibitors of Biological Pathways”, Science 285:591-595 (1999).
Oskouian, B. et al., “YAP1 Confers Resistance to the Fatty Acid Synthase Inhibitor Cerulenin Through the Transporter Flr1p inSaccharomyces cerevisiae”, Mol. Gen. Genet. 261:346-53 (1999).
Penalva, Miguel A. et al., “The Optimization of Penicillin Biosynthesis in Fungi”, Trends Biotechnol. 16

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