Radiant energy – Ionic separation or analysis – Methods
Reexamination Certificate
2007-11-20
2007-11-20
Kim, Robert (Department: 2881)
Radiant energy
Ionic separation or analysis
Methods
C250S288000, C702S076000
Reexamination Certificate
active
11144178
ABSTRACT:
A method of analyzing data from a mass spectrometer for a data dependent acquisition is described. In an embodiment of this method, mass spectral scans are taken of a sample eluted from a liquid chromatography column. An extracted ion chromatogram (XIC) is then created for each m/z data point of the mass spectral scans and the XIC for each m/z data point are correlated to a model function, such as a monotonically increasing function, or the first half of a gaussian function, to obtain a XIC correlation value. A weighting function is then applied to the XIC correlation value to obtain a current weighted intensity. The current weighted intensity for each m/z point is used to reconstruct a weighted mass spectrum, which is then used to make a real-time decision for the data dependent acquisition. In an embodiment, the data dependent acquisition is the performance of tandem mass spectrometry.Embodiments of the invention also describe a sample processing apparatus for the data dependent acquisition and a computer readable medium that provides instructions to the sample processing apparatus to perform the method described above.
REFERENCES:
patent: 5481476 (1996-01-01), Windig
patent: 5672869 (1997-09-01), Windig et al.
patent: 6002986 (1999-12-01), Mito
patent: 2004/0181351 (2004-09-01), Thompson et al.
patent: 2004/0191351 (2004-09-01), Kim et al.
patent: 2004/0251409 (2004-12-01), Le Blanc
patent: 2006/0284069 (2006-12-01), Le Blanc
Andreev et al., “A Universal Denoising and Peak Picking Algorithm for LC-MS Based on Matched Filtration in the Chromatographic Time Domain,” Anal. Chem., vol. 75 ( No. 22), p. 6314-6326, (Nov. 15, 2003).
Kohli et al., “An Alternative Sampling Algorithm for Use in Liquid Chromatography/Tandem Mass Spectrometry Experiments,” Rapid Commun. Mass Spectrom., Wiley InterScience, p. 589-596, (2005).
Wenner et al., “Factors That Affect Ion Trap Data-Dependent MS/MS in Proteomics,” Amer. Soc. Mass Spectrom., Elsevier Inc., p. 150-157, (2004).
Hemenway Eric
Hemenway Tina A.
Senko Michael W.
Allred David E.
Blakely , Sokoloff, Taylor & Zafman LLP
Johnston Phillip A.
Kim Robert
Thermo Finnigan LLC
LandOfFree
Methods for improved data dependent acquisition does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for improved data dependent acquisition, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for improved data dependent acquisition will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3845483