Methods for immobilizing nucleic acids on a gel substrate

Details Methods for immobilizing nucleic acids on a gel substrate Methods for immobilizing nucleic acids on a gel substrate

1997-07-17

1999-11-09

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2531, 536 2532, 428 415, C07H 2100, C07H 2104, C08F12056

Patent

active

059817348

ABSTRACT:
A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.

REFERENCES:
Fatty et al. Nucl. Acids Res. 21(8): 1819-1826, 1993.
Timofeev, et al. Regioselective immobilizaton of short oligonucleotides to acrylic copolymer gels, vol. 24, pp. 3142-3148. (1996 Nucleic Acids Research).
Proudnikov, et al. "Chemical methods of DNA and RNA fluorescent labeling". Vol. 24, pp. 4535-4542. (1996 Nucleic Acids Research).

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