Methods for identifying the DQw3.2 allele associated with increa

Details Methods for identifying the DQw3.2 allele associated with increa Methods for identifying the DQw3.2 allele associated with increa

1989-03-13

1993-03-23

Saunders, David A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 724, 435 79, 435948, 436506, 436518, 436530, 436531, 436548, 436800, 436804, 436809, 436811, 436821, 935110, G01N 33543, G01N 33564, G01N 33577

Patent

active

051963086

ABSTRACT:
Methods for identifying individuals at increased risk of diabetes are disclosed. The methods disclosed utilize the discovery of the DQw3.2 variant, which identifies a specific allelic polymorphism at a sinle gene locus. One preferred method utilizes a labeled probe to detect the DQw3.2 allele. This method involves estimating the size of the hybridizable DNA fragment generated by a specific restriction endonuclease and therefrom determining the presence of the allele. A second preferred method involves the serologic detection of the DQw3.2 allele. Within this method, immunocomplexes formed between two different MAb's and separate portions of a cell collection are detected and the presence or absence of the allele determined.

REFERENCES:
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Duquesnoy, R. J. et al. 1984. Antigen Report: HLA-DQw3. In: Histocompatibility Testing 1984. E. D. Albert et al. Editors Springer-Verlag, Berlin. pp. 209-211.
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