Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-03-04
1999-09-21
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435691, 435 701, 4352404, 4353201, 530350, C12Q 168, C12Q 170, C12P 2100, C12N 1570
Patent
active
059552751
ABSTRACT:
Methods for identifying nucleic acid sequences that affect a cellular phenotype are disclosed. The method uses a reporter gene whose level of expression correlates with the phenotype in conjunction with a method or device for measuring the level of reporter expression. An expression library is introduced into the cells, and those cells exhibiting changes in reporter expression level are selected. Expression library inserts from the selected cells are isolated, thereby providing a sub-library enriched for sequences that affect the phenotype reflected by the reporter. Further rounds of sub-library introduction and cell selection may be carried out to provide additional enrichment Sequences identified using this method may be used to ascertain the identity of additional molecules involved in generating the cellular phenotype.
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Arcaris, Inc.
Fredman Jeffrey
Shuster Michael J.
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