Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-08-17
1993-12-14
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
052701638
ABSTRACT:
The SELEX (Systematic Evolution of Ligands by EXponential Enrichment) method is disclosed for the identification of nucleic acid ligands. A candidate mixture of single stranded nucleic acids having regions of randomized sequence is contacted with a target compound and those nucleic acids having an increased affinity to the target are partitioned from the remainder of the candidate mixture. The partitioned nucleic acids are amplified to yield a ligand enriched mixture.
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Oliphant, Brandl, and Struhl (1989) Mol. and Cell Biology Jul. 1989 pp. 2944-2949, Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Binding Sites From Random-Sequence Oligonucleotides; Analysis of Yeast GCN4 Protein.
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Thiesen and Bach (1990) Nucleic Acids Research 18:3203-3209, entitled: Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein.
Gold Larry
Tuerk Craig
Parr Margaret
University Research Corporation
Zitomer Stephanie W.
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