Methods for identifying inhibitors of fungal pathogenicity

Details Methods for identifying inhibitors of fungal pathogenicity Methods for identifying inhibitors of fungal pathogenicity

1994-02-28

1997-03-25

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 6, 435 15, 435 29, C12Q 118, C12Q 102, C12Q 168, C12Q 148

Patent

active

056143770

ABSTRACT:
Methods of detecting inhibitors of fungal pathogenicity are disclosed. These methods are based on the discovery that pathogenicity is diminished when chitin synthase III is inhibited. The inhibitors of fungal pathogenicity are detected with mutant strains of Saccharomyces cerevisiae or other mutant fungal strains, whose mutation causes diminished growth of the fungus when combined with the inhibition of chitin synthase III.

REFERENCES:
E. Cohen, "Chitin Synthesis and Degradation as Targets for Pesticide Action," Archives of Insect Biochemistry and Physiology, 22:245-261 (1993).
C. E. Bulawa, "CSD2, CSD3, and CSD4, Genes Required for Chitin Synthesis in Saccharomyces cerevisiae: the CSD2 Gene Product is Related to Chitin Synthases and to Developmentally Regulated Proteins in Rhizobium Species and Xenopus laevis," Molecular and Cellular Biology, 12(4):1764-1776 (Apr. 1992).
D. J. Adams and G. W. Gooday, "Chitin Synthesis as a Target-Current Progress," in Systemische Fungizide und Antifungale Verbindungen, pp. 39-45 (1983).
A. R. Bowen, et al., "Classification of Fungal Chitin Synthases,"proc. Natl. Acad. Sci. USA, 89:519-523 (Jan. 1992).
M. Henar Valdivieso, et al., "A Gene Required for Activity of Chitin Synthase 3 in Saccharomyces Cerevisiae," The Journal of Cell Biology, 114(1):101-110 (Jul. 1991).
R.F. Hector et al., "A 96-Well Epifluorescence Assay For Rapid Assessment of Compounds Inhibitory to Candida Spp.", J. Clin. Microbiol. 24(4) 620-624 Oct. 1986.
P.J. McCarthy et al., "Mechanism of Action of Nikkomycin and the Peptide Transport System of Candida albicans", 131(4) 775-780 1985.
P. Orlean, "Two Chitin Synthetases in Saccharomyces cerevisiae", J. Biol. Chem. 262(12) 5732-5739 Apr. 1989.

Affiliated with

Also associated with

Rating

Methods for identifying inhibitors of fungal pathogenicity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for identifying inhibitors of fungal pathogenicity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for identifying inhibitors of fungal pathogenicity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2202358