Methods for identifying DNA sequences for use in comparison of D

Details Methods for identifying DNA sequences for use in comparison of D Methods for identifying DNA sequences for use in comparison of D

1999-09-17

2000-11-21

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 231, 536 242, C12Q 168, C07H 2102

Patent

active

061501123

ABSTRACT:
A general method for screening genomic or cDNA, or fragments and mixtures thereof, involves sample simplification by the generation of subsets and then subjecting the subsets to a modified mismatch scanning procedure that eliminates DNA having single stranded breaks after a MutSLH cleavage. The methods are particularly useful in human population isolates, including identification of identical-by-descent sequences, genomic comparisons of two or more individuals, and genomic comparisons of two populations of individuals, for the identification of sequences of low polymorphism.

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McAllister et al "ERnrichment for Loci Identical-by Descent between pairs of Mouse or Human genomes by Genomic Mismatch Scanning" Genomics,vol. 47, pp. 7-11, Jan. 1998.
Riley et al "A novel, rapid method for the isolation of terminal sequences from YAC clones" Nucleic Acid Research, vol. 18, No.10, pp. 2887-2890, 1990.
Cheung et al "Genomic Mismatch Scanning: Applications to linkage and linkage disequilibrium analysis" Am. J. of Human Genetics, vol. 61, No. 4, Suppl. pp. A271, Oct. 1997.
Nelson, Nature Geneticcs (1993), vol. 4: 11-18.
Prasher & Weissman Proc. Nat. Acad. USA (1996)93: 659-663.
Geung et al. Nature Genetics (1998), 18: 225-230.

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