Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-09-05
2006-09-05
Housel, James (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S005000, C435S006120, C435S091200, C435S456000, C435S235100, C435S069100, C424S184100, C424S207100, C424S208100, C530S300000, C530S350000
Reexamination Certificate
active
07101676
ABSTRACT:
The present invention relates to methods of identifying a molecule from a library of molecules that inhibits binding of human immunodeficiency virus nucleocapsid 7 polypeptide (NCp7) to an oligonucleotide which comprises admixing an NCp7 polypeptide with at one labeled HIV-1 psi-site oligonucleotide and an amount of the molecule to be tested under binding conditions; and determining the amount of oligonucleotide bound to the NCp7 polypeptide, wherein a decrease in the amount of oligonucleotide bound in the presence of the molecule compared with the amount of oligonucleotide bound in the absence of the molecule indicates that the molecule inhibits binding of NCp7 polypeptide to the oligonucleotide.
REFERENCES:
patent: 5652260 (1997-07-01), Kun et al.
patent: 5652367 (1997-07-01), Kun et al.
patent: 5668291 (1997-09-01), Domagala et al.
patent: 5670518 (1997-09-01), Kun et al.
patent: 5733921 (1998-03-01), Bolton et al.
patent: 5734081 (1998-03-01), Domagala et al.
patent: 5753674 (1998-05-01), Kun et al.
patent: 5783384 (1998-07-01), Verdine
patent: 5877185 (1999-03-01), Kun et al.
patent: 5889034 (1999-03-01), Bolton et al.
patent: 5929114 (1999-07-01), Domagala et al.
patent: 6001863 (1999-12-01), Bolton et al.
patent: 6004978 (1999-12-01), Kun et al.
patent: 6008190 (1999-12-01), Meade et al.
patent: 6046228 (2000-04-01), Rice et al.
patent: 6133270 (2000-10-01), Bolton et al.
patent: 6225323 (2001-05-01), Yatscoff et al.
patent: 6242478 (2001-06-01), Welker et al.
patent: WO 9609406 (1996-03-01), None
Tummino et al. The in vitro ejection of zinc from human immunodeficiency virus (HIV) type 1 nucleocapsid protein by disulfide benzamides with cellular anti-HIV activity. Proc. Natl. Acad. Sci. Feb. 1996, vol. 93, pp. 969-973.
Domagala et al. A new class of anti-HIV agents targeted toward the nucleocapsid NCp7: the 2,2′-dithiobisbenzamides. Bioorg. Med. Chem. 1997, vol. 5(3) 569-579.
Domagala et al. 2,2′-dithiobisbenzamides and 2-benzisothiazolones: two new classes of antiretroviral agents: SAR and mechanistic consideration. Drug Design Discovery, 1997, vol. 15(1), 49-61.
Rice et al. Inhibition of HIV-1 infectivity by zinc-ejecting aromatic C-nitroso compounds. Nature, vol. 361, 1993, pp. 473-475.
Rice et al. The site of antiviral action of 3-nitrosobenzamide on the infectivity process of human immunodeficiency virus in human lymphocytes. Proc. Natl. Acad. Sci. vol. 90, 1993, pp. 9721-9724.
Rice et al. Inhibitors of HIV nucleocapsid protein zinc fingers as candidates for the treatment of AIDS. Science. vol. 270, 1995, 1194-1197.
Tummino et al. The human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein zinc ejection activity of disulfide benzamides and benzisothiazolones: correlation with anti-HIV and virucidal activities. Antimicrobial Agents and Chemotherapy. vol. 41 (2), 1997, pp. 394-400.
Aldovini A, and Young RA, Mutations of RNA and Protein Sequences Involved in Human Immunodeficiency Virus Type 1 Packaging Result in Production of Noninfectious Virus, J Virol May 1990, 64(5):1920-1926.
Altschul, et al., Basic Local Alignment Search Tool, (1990) J. Mol. Biol. 215:403-410.
Amara et al., Control of a Mucosal Challenge and Prevention of AIDS by a Multiprotein DNA/MVA Vaccine, Science Apr. 6, 2001;292(5514): 69-74.
Amarasinghe et al., NMR Structure of the HIV-1 Nucleocapsid Protein Bound to Stem-Loop SL2 of the Ψ-RNA Packaging Signal. Implications for Genome Recognition, J Mol Biol Aug. 11, 2000;301(2):491-511.
Basrur et al., Inactivation of HIV-1 Nucleocapsid Protein P7 by Pyridinioalkanoyl Thioesters, J Biol Chem May 19, 2000;275(20):14890-14897.
Berg, Potential Metal-Binding Domains in Nucleic Acid Binding Proteins, Science Apr. 25, 1986; 232(4749):485-487.
Darlix, et al., First Glimpses at Structure-function Relationships of the Nucleocapsid Protein of Retroviruses, J Mol Biol Dec. 8, 1995; 254(4):523-537.
De Guzman et al., Structure of the HIV-1 Nucleocapsid Protein Bound to the SL3 Ψ-RNA Recognition Element, Science Jan. 16, 1998; 279(5349):384-388.
de Rocquigny H. et al., First Large Scale Chemical Synthesis of the 72 Amino Acid HIV-1 Nucleocapsid Protein NCp7 in an Active Form, Biochem Biophys Res Commun Oct. 31, 1991;180(2):1010-8.
Dorfman et al., Mapping of Functionally Important Residues of a Cysteine-Histidine Box in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein, J Virol Oct. 1993;67(10):6159-69.
Gorelick, et al., Strict Conservation of the Retroviral Nucleocapsid Protein Zinc Finger is Strongly Influenced by its Roles in Viral Processes: Characterization of HIV-1, Particles Containing Mutant Nucleocapsid Zinc-Coordinating Sequences, Virology Mar. 30, 1999;256(1):92-104.
Guo, J, et al., Zinc Finger Structures in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Facilitate Efficient Minus- and Plus-Strand Transfer, J Virol Oct. 2000;74(19):8980-8.
Huang M, et al., Anti-HIV Agents That Selectively Target Retroviral Nucleocapsid Protein Zinc Fingers without Affecting Cellular Zinc Finger Proteins, J Med Chem Apr. 23, 1998;41(9):1371-81.
Maynard AT, et al., Reactivity of the HIV-1 nucleocapsid protein p7 zinc finger domains from the perspective of density-functional theory, Proc Natl Acad Sci USA Sep. 29, 1998;95(20);11578-83.
Maynard AT and Covell DG, Reactivity of Zinc Finger Cores; Analysis of Protein Packing and Electrostatic Screening, J Am Chem Soc Feb. 14, 2001;123(6):1047-58.
McDonnell NB, et al., Zinc Ejection as a New Rationale for the Use of Cystamine and Related Disulfide-Containing Antiviral Agents in the Treatment of AIDS, J Med Chem Jun. 20, 1997;40(13):1969-76.
Ramboarina S. et al., Structural Investigation on the Requirement of CCHH Zinc Finger Type in Nucleocapsid Protein of Human Immunodeficiency Virus 1, Biochemistry Jul. 27, 1999;38(30):9600-7.
Rice WG, et al., Inhibition of Multiple Phases of Human Immunodeficiency Virus Type 1.
South TL, et al., The Nucleocapsid Protein Isolated from HIV-1 Particles Binds Zinc and Forms Retroviral-Type Zinc Fingers, Biochemistry Aug. 28, 1990;29(34):7786-9.
South TL and Summers MF, Zinc Fingers, Adv Inorg Biochem 1990;8:199-248.
Takahashi K, et al., Two Basic Regions of NCp7 Are Sufficient for Conformational Conversion of HIV-1 Dimerization Initiation Site from Kissing-loop Dimer to Extended-duplex Dimer, Biol Chem Aug. 17, 2001;276(33):31274-8.
Turpin JA, et al., Synthesis and Biological Properties of Novel Pyridinioalkanoyl Thiolesters (PATE) as Anti-HIV-1 Agents That Target the Viral Nucleocapsid Protein Zinc Fingers, J Med Chem Jan.14, 1999;42(1):67-86.
Vuilleumier C, et al., Nucleic Acid Sequence Discrimination by the HIV-1 Nucleocapsid Protein NCp7: A Fluorescence Study, Biochemistry Dec. 21, 1999;38(51);16816-25.
Williams MC, et al., Mechanism for nucleic acid chaperone activity of HIV-1 nucleocapsid protein revealed by single molecule stretching, Proc Natl Acad Sci USA May 22, 2001;98(11):6121-6.
Rice, et al., Inhibitors of HIV Nucleocapsid Protein Zinc Fingers as Candidates for the Treatment of AIDS, Science Nov. 17, 1995;270(5239): 1194-97.
Rice, et al. The Site of Antiviral Action of 3-nitrosobenzamide on the Infectivity Process of Human Immunodeficiency Virus in Human Lymphocytes, Proc. Natl. Acad. Sci. USA Oct. 15, 1993; 90(20): 9721-24.
Tummino, et al. The vitro Ejection of Zinc From Human Immunodeficiency Virus (HIV) typ 1 Nucleocapsid Protein by Disulfide Benzamides with Cellular Anti-HIV Activity, Proc. Natl. Acad. Sci. USA Feb. 6, 1996;93(3): 969-73.
Liitsola, K. et al., “Genetic Characterization of HIV-1 Strains in the Baltic Countries and Russia,”Scand J. Infect. Dis. 1996; 28: 537-541.
International Search Report for Application No. PCT/US03/00801. Mailing Date Jun. 22, 2005; Total 6 pages.
Buechter Douglas
Hou Xiaohong
Marlor Christopher W.
Rice William G.
Yang Wengang
Housel James
Le Emily M.
Wilmer Cutler Pickering Hale & Dorr LLP
LandOfFree
Methods for identifying compounds which inhibit binding of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for identifying compounds which inhibit binding of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for identifying compounds which inhibit binding of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3563316