Methods for identifying compounds that bind to PUR protein

Details Methods for identifying compounds that bind to PUR protein Methods for identifying compounds that bind to PUR protein

1995-06-07

1997-09-30

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 723, 435 771, 435 793, 530300, 530358, 935 39, 935 41, G01N 3353, G01N 33574, C07K 200, C12N 1500

Patent

active

056724799

ABSTRACT:
The present invention relates to the PUR protein, nucleotide sequences and expression vectors encoding PUR, and to methods for inhibiting PUR activity. Inhibitors of PUR activity may be used to treat hyperproliferative diseases such as cancer.

REFERENCES:
Wobbe et al., 1987, "Replication of Simian Virus 40 Origin-containing DNA in vitro With Purified Protein" Proc. Natl. Acad. Sci. USA 84:1834-1838.
Traut, W. and Fanning, E., 1988, "Sequence Specific Interactions Between a Cellular DNA-binding Protein and the Simian Virus 40 Origin of DNA Replication" Mol. Cell. Biol. 8:903-911.
Vassilev, L. and Johnson, E.M. 1989, "Mapping Initiation Sites of DNA Replication in vivo Using Polymerase Chain Reaction Amplification of Nascent Strand Segments" Nucl. Acids Res. 17:7693-7705.
Caddle, M.S. et al., 1990, "Intramolecular DNA Triplexes, Bent DNA and DNA Unwinding Elements in the Initiation Region of an Amplified Dihydrofolate Reductase Replicon" J. Mol. Biol. 211:19-33.
Vassilev, L. and Johnson, E.M., 1990, "An Initiation Zone of Chromosomal DNA Replication Located Upstream of the c-myc Gene in Proliferating HeLa Cells" Mol. Cell. Biol. 10:4899-4904.
Dailey, L. et al., 1990, "Purification of RIP60 and RIP100, Mammalian Proteins with Origin Specific DNA-binding and ATP-Dependent DNA Helicase Activity" Mol. Cell. Biol. 10:6225-6235.
Caddle, M.S. et al., 1990, "RIP60, a Mammalian Origin-Binding Protein, Enhances DNA Bending Near the Dihydrofolate Reductase Origin of Replication" Mol. Cell. Biol. 10:6236-6243.
Hofmann, J. F.-X. and Gasser, S.M., 1991, "Identification and Purification of a Protein that Binds the Yeast ARS Consensus Sequence" Cell 64:951-960.
Gutierrez et al., 1991, "Mechanism of Stimulation of DNA Replication by Bacteriophage .o slashed.29 Single-Standed DNA-binding Protein p5" J. Biol. Chem., 266:2104-2111.
Erdile et al., 1991, "Characterization of a cDNA Encoding the 70-kDa Single-stranded DNA-binding Subunit of Human Replication Protein A and the Role of the Protein in DNA Replication" J. Biol. Chem., 266:12090-12098.
Bergemann, A.D. and Johnson, E.M., 1992, "The HeLa PUR Factor Binds Single-Stranded DNA at a Specific Element Conserved in Gene Flanking Regions and Origins of DNA Replication" Mol. Cell. Biol. 12:1257-1265.
Bergemann, A.D. and Johnson, E.M., 1992, "Sequence of cDNA Comprising the Human pur Gene and Sequence-Specific-Single-Stranded-DNA-Binding Properties of the Encoded Protein" Mol. Cell. Biol. 12:5673-5682.

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