Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2005-04-19
2010-10-05
Martinell, James (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S018000
Reexamination Certificate
active
07807400
ABSTRACT:
The present invention provides a method of identifying a compound having the ability to modulate the guanine nucleotide exchange cycle of a Ras superfamily GTPase, comprising: a) contacting the compound with a guanine nucleotide exchange factor and a GTPase and obtaining a baseline fluorescence measurement; b) contacting the guanine nucleotide exchange factor and the GTPase without the compound and obtaining a baseline fluorescence measurement; c) adding a fluorophore-conjugated GTP to the components of (a) and (b), respectively; d) obtaining fluorescence measurements of the respective components of (c) over time; e) subtracting the respective baseline fluorescence measurements of (a) and (b) from each fluorescence measurement of (d); and f) comparing the resulting fluorescence values of (e), wherein a decrease or increase in the rate of fluorescence change with the compound as compared with the rate of fluorescence change without the compound identifies a compound having the ability to modulate the guanine nucleotide exchange cycle of a Ras superfamily GTPase. Further provided are compounds of the invention and pharmaceutical compositions comprising compounds of the invention useful for the treatment of cancer and neurological disorders.
REFERENCES:
patent: 2006/0263772 (2006-11-01), Shoemaker et al.
International Search Report for PCT/US05/13444, dated Feb. 14, 2007.
Stephen et al. “The nucleocapsid protein as a target for novel anti-HIV drugs” www.currentdrugdiscovery.com, pp. 33-36, 2003.
Rojas et al. “Established and Emerging Fluorescence-Based Assays for G-Protein Function: Ras-Superfamily GTPases”Combinatorial Chemistry & High Throughput Screening6(4):409 (Jun. 2003).
Rojas Rafael
Sondek John
Martinell James
Myers Bigel Sibley & Sajovec P.A.
The University of North Carolina at Chapel Hill
LandOfFree
Methods for identifying chemical modulators of Ras... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for identifying chemical modulators of Ras..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for identifying chemical modulators of Ras... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4203284