Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1998-05-12
1999-10-26
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 6, 435 29, C12Q 118
Patent
active
059726407
ABSTRACT:
Antimitotic and antifungal agents are identified in screens comprising the steps of (a) contacting in culture first and second cells with an agent not previously known to be associated with mitotic arrest, wherein said cells differ in that the second cell has a functionally disrupted mitotic checkpoint; and (b) detecting a resultant sensitivity of the cells to the agent, whereby a higher sensitivity of the second cell identifies the agent as an antimitotic agent. The functionally disrupted mitotic checkpoint may be provided by a genetic mutation in a mad or bub gene, wherein mitotic arrest effects death of the cell. Yeast provide particularly useful cells for high throughput screens, including yeast transgenic in a human tubulin gene.
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Tavormina P., Genetic Analysis of Mitotic Checkpoint Controls in the Yeast Saccharomyces cerevisiae, Dissertation Abstracts Int, 59(2B)513, Aug. 1998.
Chen Ray-Huei, Association of Spindle Assembly Checkpoint Component XMAD2 with Unattached Kinetochores, Science 274, Oct. 1996.
Hwang L., A Novel Yeast Screen for Mitotic Arrest Mutants Identifies DOC1, a New Gene Involved in Cyclin Proteolysis. Molecular Biology of the Cell vol. 8, 1877-1887, Oct. 1997.
Barnes Georjana
Drubin David G.
Gitomer Ralph
Osman Richard Aron
The Regents of the University of California
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