Methods for identifying and using MarR family polypeptide...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Reexamination Certificate

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08039221

ABSTRACT:
Methods for identifying MarR family inhibiting compounds are described. The methods include the use of computer aided rational based drug design programs and three dimensional structures of MarR family polypeptides.

REFERENCES:
patent: 5817793 (1998-10-01), Levy
patent: 6346391 (2002-02-01), Oethinger
patent: 6391545 (2002-05-01), Levy
patent: 6448006 (2002-09-01), Levy
patent: 7075582 (2006-07-01), Alekshun
patent: WO 03/006626 (2003-01-01), None
Alekshun, et al. The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 A resolution.: Nat Struct Biol. Aug. 2001; 8(8):710-4.
Alekshun, M.N. et al. “Regulation of charomosomally mediated multiple antibiotic resistance: the mar regulon.” Antimicrob. Agents Chemother. Oct. 1997;41(10):2067-75.
Alekshun, M.N. et al. “Characterization of MarR superrepressor mutants.” J. Bacteriol. May 1999;181(10):3303-6.
Alekshun, M.N. et al. “Alteration of the repressor activity of MarR, the negative regulator of theEscherichia colimarRAB locus, by multiple chemicals in vitro .” J. Bacteriol. Aug. 1999; 181(15):4669-72.
Alekshun, M.N. et al. “The mar regulon: multiple resistance to antibiotics and other toxic chemicals.” Trends Microbiol. Oct. 1999;7(10):410-3.
Alekshun, M.N. et al., “Mutational analysis of MarR, the negative regulator of marRAB expression inEscherichia coli, suggests the presence of two regions required for DNA binding.” Mol. Microbiol. Mar. 2000;35(6):1394-404.
Barbosa, T.M. et al. “Differential expression of over 60 chromosomal genes inEscherichia coliby constitutive expression of MarA.” J. Bacteriol. Jun. 2000;182(12):3467-74.
Brooun, A. et al. “Purification and ligand binding of EmrR, a regulator of a multidrug transporter.” J. Bacteriol. Aug. 1999;181(16):5131-3.
Drenth, J. “Chapter 1: Crystallizing a protein.” from Principles of Protein X-ray Crystallography pp. 1-18, 1994 Springer-Verlag New York, Inc.
Gajiwala, K.S. et al. “Winged helix proteins.” Curr. Opin. Struct. Biol. Feb. 2000;10(1):110-6.
Gajiwala, K.S. et al. “Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding.” Nature Feb. 24, 2000;403(6772):916-21.
Geneseq.sub.—101002 database, AAR4977, dated Oct. 14, 1994; Result #1.
Kem. W.V. et al. “Non-target gene mutations in the development of fluoroquinolone resistance inEscherichia coli.” Antimicrob. Agents Chemother. Apr. 2000;44(4):814-20.
Koutsolioutsou, A. et al. “A soxRS-constitutive mutation contributing to antibotic resistance in a clinical isolate ofSalmonella enterica(Serovar typhimurium).” Antimicrob. Agents Chemother. Jan. 2001;45(1):38-43.
Linde, H.J. et al. “In vivo increase in resistance to ciprofloxacin inEscherichia coliassociated with deletion of the C-terminal part of MarR.” Antimicrob. Agents Chemother. Jul. 2000;44(7):1865-8.
Maneewannakul, K. et al. “Identification for mar mutants among quinolone-resistant clinical isolates ofEscherichia coli.” Antimicrob. Agents Chemother. Jul. 1996;40(7):1695-8.
Martin, R.G. et al. “Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences.” Proc. Natl. Acad. Sci. U.S.A. Jun. 6, 1995;92(12):5456-60.
Martin, R.G. et al. “Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator inEscherichia coli.” J. Bateriol. Apr. 1996;178(8):2216-23.
Nikaido, H. “Multiple antibiotic resistance and efflux.” Curr. Opin. Microbiol. Oct. 1998;1(5):516-23.
Oethinger, M. et al. “Overexpression of the marA or soxS regulatory gene in clinical topoisomerase mutants ofEscherichia coli.” Antimicrob. Agents Chemother. Aug. 1998;42(8):2089-94.
Pohl, E. et al. “Motion of the DNA-binding domain with respect to the core of the diphtheria toxin repressor (DtxR) revealed in the crystal structures of apo- and holo-DtxR.” J. Biol. Chem. Aug. 28, 1998;273(35):22420-7.
Providenti, M.A. et al. “Indentification and functional characterization of CbaR, a MarR-like modulator of the cbaABC-encoded chlorobenzoate catabolism pathway.” Applied and Environmental Microbiology Aug. 2001: 67(8):3530-41.
Randall, L.P. et al. “Multiple antibiotic resistance (mar) locus inSalmonella entericaserovar typhimurium DT104.” Applied and Experimental Microbiology. Mar. 2001 ;67(3):1190-1197.
Sulavik, M.C. et al. “The MarR repressor of the multiple antibiotic resistance (mar) operon inEscherichia coli: prototypic member of a family of bacterial regulatory proteins involved in sensing phenolic compounds.” May 1995; 1(4):436-446.
Sulavik, M.C. et al. “TheSalmonella typhimuriummar locus: molecular and genetic analyses and assessment of its role in virulence.” J. Bateriol. Mar. 1997;179(6):1857-66.
White, A. et al. “Structure of the metal-ion-activated diphtheria toxin repressor/tox operator complex.” Nature Jul. 30, 1998;394(6692):502-6.
Zheng, N. et al. “Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DP.” Genes Dev. Mar. 15, 1999;13(6):666-74.
Ziha-Zarifi, I. et al. “In vivo emergence of multidrug-resistant mutants ofPseudomonas aeruginosaoverexpressing the active efflux system MexA-MexB-OprM.” Antimicrob. Agents Chemother. Feb. 1999;43(2)287-91.

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