Methods for identifying and isolating variant T4 DNA polymerases

Details Methods for identifying and isolating variant T4 DNA polymerases Methods for identifying and isolating variant T4 DNA polymerases

1995-06-06

1997-08-26

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

530358, 935 79, 935 82, C12Q 170

Patent

active

056609809

ABSTRACT:
Method for identifying and isolating variant T4 DNA polymerase by isolating and selecting for T4 strains having variant DNA polymerase defective in DNA replication, and at least one additional mutation which corrects or compensates for said defect in DNA replication; identifying the additional mutation(s) and introducing the mutation(s) into T4 phage or T4 DNA polymerase expression vectors.

REFERENCES:
Reha-Krantz et al., Proc. Natl. Acad. Sci., 88:2417-2421 Mar. 1991.

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