Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-06-07
2005-06-07
Woitach, Joseph (Department: 1632)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C435S001100, C436S518000
Reexamination Certificate
active
06903196
ABSTRACT:
This invention provides novel methods of and kits for labeling and isolating lumen-exposed molecules, particularly polypeptides which are expressed in a tissue-specific or organ-specific manner. The methods and kits can be used to isolate molecules exposed on the luminal side of cells lining vascular, ductal, sinus, respiratory, fascial, and other perfusible tissue spaces.
REFERENCES:
patent: 5218700 (1993-06-01), Beechick
patent: 5610008 (1997-03-01), Schnitzer et al.
patent: 5660827 (1997-08-01), Thorpe et al.
patent: 5776427 (1998-07-01), Thorpe et al.
patent: 5776770 (1998-07-01), Schnitzer et al.
patent: 5855866 (1999-01-01), Thorpe et al.
patent: 5914127 (1999-06-01), Schnitzer et al.
patent: 5914312 (1999-06-01), Axworthy et al.
patent: 5948624 (1999-09-01), Rothschild et al.
patent: 5965132 (1999-10-01), Thorpe et al.
patent: 5994519 (1999-11-01), Osbourn et al.
patent: 6004554 (1999-12-01), Thorpe et al.
patent: 6068829 (2000-05-01), Ruoslahti et al.
patent: 6136311 (2000-10-01), Bander
patent: WO97/04748 (1997-02-01), None
patent: WO 98/39469 (1998-09-01), None
patent: WO 00/07625 (2000-02-01), None
patent: WO01/71359 (2001-09-01), None
Pierce Chemical Company, 1994, 2600002 94CAT, Pierce Life Science & Analytical Research Products Catalog & Handbook.
Chen, et al. 1998, Determination of external loop topology in the serotonin transporter by site-directed chemical labeling,Journal of Biological Chemistry,273(20):12675-12681.
Kandror, et al. 1999, Insulin regulation of protein traffic in rat adipose cells.Journal of Biological Chemistry,274(36):25210-25217.
Mouton, et al. 1982. A reagent for covalently attaching biotin to proteins via a cleavable connector arm.Archives of Biochemistry, 218(1):101-108.
International Search Report from co-pending PCT/US01/08705 dated May 21, 2002.
Huwyler et al, “Brain Drug Delivery of Small Molecules Using Immunoliposomes”, Proc. Natl. Acad. Sci. USA, Nov. 1996, vol. 93, pp. 14164014164.
Huwyler et al, “Receptor Mediated Delivery of Daunomycin Using Immunoliposome: Oharmacokinetics and Tissue Distribution in the Rat”, The Journal of Pharmacology and Experimental Therapeutics, 1997, vol. 282, No. 3, pp. 1541-1546.
Hastie et al., Identification of Surface Components of Mammalian Respiratory Tract CiliaCell Motility and the Cytoskeleton17:317-328 (1990).
Schnitzer et al., Separation of Caveolae from Associated Microdomains of GPI-Anchored ProteinsScience269:1435-1439 (Sep. 1995).
Dentler William L., Nonradioactive Methods for Labeling and Identifying Membrane Surface ProteinsMethods in Cell Biology47:407-411 (1995).
Schnitzer et al., Caveolae from luminal plasmalemma of rat lung endothelium: Microdomains enriched in caveolin, Ca2+-ATPase, and inositol trisphosphate receptorProc. Natl. Acad. Sci.92:1759-1763 (Feb. 1995).
Dentler, William L., Identification ofTetrahymenaCiliary Surface Proteins Labeled with Sulfosuccinimidyl 6-(Biotinamido) Hexanoate and Concanavalin A and Fractionated with Triton X-114J. Protozool.39(3):368-378 (May-Jun. 1992).
Levy-Toledano et al. Streptavidin Blotting: A Sensitive Technique to Study Cell Surface Proteins; Application to Investigate Autophosphorylation and Endocytosis of Biotin-Labeled Insulin ReceptorsEndocrinology133(4):1803-1808 (Oct. 1993).
Bush et al., Selective isolation of individual cell surface proteins from tissue culture cells by a cleavable biotin labelEuropean Journal of Cell Biology50:257-262 (Dec. 1989).
De La Fuente et al., Biotinylation of membrane proteins accessible via the pulmonary circulation in normal hyperoxic ratsAm. J. Physiol.272(3) Part 1:L461-L470 (Mar. 1997).
Garlanda et al., Heterogeneity of Endothelial CellsArteriosclerosis, Thrombosis and Vascular Biology17(7):1193-1202 (Jul. 1997).
Lamszus et al., Isolation and Culture of Human Neuromicrovascular Endothelial Cells for the Study of Angiogenesis In VitroJournal of Neuroscience Research55:370-381 (Feb. 1999).
Craig et al., Endothelial Cells from Diverse Tissues Exhibit Differences in Growth and MorphologyMicrovascular Research55:65-76 (Jan. 1998).
Pasqualini, R., Vascular targeting with phage peptide librariesThe Quarterly Journal of Nuclear Medicine43(2):159-162 (Jun. 1999).
Pasqualini et al., Organ targeting in vivo using phage display peptide librariesNature380(28):364-366 (Mar. 1996).
Rajotte et al., Molecular Heterogeneity of the Vascular Endothelium Revealed by In Vivo Phage DisplayJournal of Clinical Investigation102(2):430-437 (Jul. 1998).
Roben Paul
Stevens Anthony C.
Utah Ventures II, L.P.
Woitach Joseph
LandOfFree
Methods for identifying and isolating tissue-specific... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for identifying and isolating tissue-specific..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for identifying and isolating tissue-specific... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3491316