Methods for identifying an essential gene in a prokaryotic...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S473000, C435S476000, C435S032000, C435S069100, C435S320100, C435S474000, C435S475000, C435S183000, C424S093200

Reexamination Certificate

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06991900

ABSTRACT:
Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.

REFERENCES:
patent: 5677170 (1997-10-01), Devine et al.
patent: 5843772 (1998-12-01), Devine et al.
patent: 6673567 (2004-01-01), Sharpe et al.
Kim, Ung-Jin et al., “Construction and Characterization of a Human Bacterial Artificial Chromosome Library,”Genomics, vol. 34, 1996, pp. 213-218.
Shizuya, Hiroaki and Kouros-Mehr, Hosein, “The Development and Applications of the Bacterial Artificial Chromosome Cloning System,” Keio Journal of Medicine, Vol. 50, No. 1, Mar. 2001, pp. 26-30.

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