Methods for identification or purification of cells containing a

Details Methods for identification or purification of cells containing a Methods for identification or purification of cells containing a

1995-05-15

1999-03-02

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 29, 435372, C12Q 132

Patent

active

058769560

ABSTRACT:
Production of a stem cell enriched cell composition using essentially non-toxic methodology comprising contacting a cell mixture with a cell-permeable, fluorescent non-polar compound that reacts upon contact with intracellular enzyme aldehyde dehydrogenase or phosphokinase found substantially exclusively in stem cells so as to form a biocompatible fluorescent product that is polar and non-permeable to the membrane of the stem cells, and isolating the cells containing the marker by fluorescent cell sorting.

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