Methods for high purity chromatographic separation of proteins h

Details Methods for high purity chromatographic separation of proteins h Methods for high purity chromatographic separation of proteins h

1992-12-16

1995-01-03

Hill, Jr., Robert J.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530324, 530399, 530416, A61K 3700

Patent

active

053788168

ABSTRACT:
Proteins having an epidermal growth factor-like binding region, such as thrombomodulin, may be recovered and purified on a anion exchange resin, such as a polyethyleneimine resin. Conditioned medium containing recombinantly produced protein is applied to the resin under conditions of pH and ionic strength selected to result in specific binding of the protein to the resin. The column is then washed to remove non-bound proteins and bound proteins other than the desired protein to be separated. The desired protein is then eluted from the column and collected in a medium. Further purification, concentration, and other treatment of the protein may be performed by conventional techniques.

REFERENCES:
Parkinson J Biol Chem 265, 12602, 1990.
Alpert J Chromatogr 185, 375 1979.
Kitagawa J Chromatogr 443, 133 1988.

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