Methods for H-phosphonate syntheis of oligonucleotides using tri

Details Methods for H-phosphonate syntheis of oligonucleotides using tri Methods for H-phosphonate syntheis of oligonucleotides using tri

1995-04-28

1997-06-17

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, C07H 102, C07H 2100

Patent

active

056398751

ABSTRACT:
New methods of synthesizing mono- and oligo-nucleoside H-phosphonates are disclosed. The methods comprise contacting a mononucleoside with phosphorous acid and triphosgene to yield the corresponding mononucleoside H-phosphonate. A similar procedure can be used to couple a first mononucleoside to a second mononucleoside or to an oligonucleotide, the method comprising contacting, in the presence of triphosgene, a mononucleoside or oligonucleotide having a free 5' hydroxyl with a mononucleoside having a 3' hydroxyl-bearing phosphorous moiety (preferably H-phosphonate).

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Alexander et al. Collect. Czech. Chem. Commun. 59:1870, 1989.

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