Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion
Patent
1998-12-11
2000-11-28
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Involving particle-mediated transfecion
800293, 800278, 8003202, 435440, 435468, 435470, A07H 100, C12N 1529, C12N 1582, C12N 1500, A01H 106
Patent
active
061538132
ABSTRACT:
A method for transforming rice plants to express heterologous DNA which involves the biolistic bombardment of mature rice seeds (embryo bearing) and results in expression of the heterologous DNA coated on the biolistic particle in the post-bombardment embryo. The heterologous DNA may be introduced as a plastid, as opposed to bare DNA. The transformed rice embryo is regenerated into a rice plantlet which can be transferred from greenhouse to field.
REFERENCES:
patent: 5141131 (1992-08-01), Miller, Jr. et al.
Cao et al., Transformation of Rice and Maize Using the Biolistic Process UCLA Symposia on Molecular and Cellular Biology, vol. 129, pp 21-33, 1990.
Christou et al., Production of Transgenic Rice (Oryza Sativa) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos, Bio/Technology, vol. 9, pp. 957-, 1991.
Riazuddin et al., Insect Resistant Transgenic Basmati Rice, Rice Biotechnology Quarterly, vol. 23, pp 7-8, 1995.
Chen Liza Ming-Ju
Reichert Nancy A.
Rudraswamy Vanishree
Fox David T.
Grunberg Anne Marie
Kelber Steven B.
Mississippi State University
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