Methods for genome identification using direct label probe compo

Details Methods for genome identification using direct label probe compo Methods for genome identification using direct label probe compo

1995-06-07

1998-08-04

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 221, 536 231, 536 243, 536 253, 536 254, 536 2532, 536 553, 935 77, 935 78, C07H 2104, C12Q 168

Patent

active

057891613

ABSTRACT:
Direct label probe compositions which counterstain genomic DNA are provided that comprise mixed DNA segments which are covalently bound to fluorophore groups through linking groups. The mixed DNA segments are derived from the total genomic DNA of a multi-chromosomal organism, especially man, and the segment mixture is approximately representative of such total genomic DNA. These probe compositions can be used concurrently or sequentially with other probe compositions. Instead of intercalating with DNA as in prior art chemical counterstains, these probe compositions hybridize with complementary segments that are present in the genomic DNA of a specimen.

REFERENCES:
patent: 5447841 (1995-09-01), Gray et al.
patent: 5491224 (1996-02-01), Bittner et al.
patent: 5506350 (1996-04-01), Bittner et al.
Schwarzacher et al., Annals of Botany 64:315-324, 1989.

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