Methods for generating resistance against CGMMV in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C800S285000, C800S265000, C800S295000, C435S419000, C435S468000

Reexamination Certificate

active

07459604

ABSTRACT:
The present invention relates to methods for generating resistance against Cucumber Green Mottle Mosaic Virus (CGMMV) in plants, in particular in plants susceptible to infection by CGMMV, such as Cucurbitaceae species, including melon, cucumber, watermelon and bottlegourd. The methods are based on the use of genetic constructs that induce post-transcriptional gene silencing and/or use a nucleotide sequence that encodes a defective variant of the replicase of CGMMV.

REFERENCES:
patent: WO 92/03539 (1992-03-01), None
patent: WO 95/04825 (1995-02-01), None
patent: WO 01/09300 (2001-02-01), None
Thomas et al. Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. (2001) The Plant Journal, vol. 25, pp. 417-425.
Waterhouse et al. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. (1998) PNAS, vol. 95, pp. 13959-13964.
Ugaki et al. THe complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA (1991) Journal of General Virology, vol. 72, pp. 1487-1495.
Shi et al. Cloning and expression of Potato virus Y HC-Pro (2005) GenBank Accession AY518285, pp. 1-2.
Tan et al. The genome structure of kyuri geen mottle mosaic tobamovirus and its comparison with that of cucumber green mottle mosaic tobamovirus. (2000) Arch. Virol.; vol. 145, pp. 1067-1079.

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