Methods for generating recombined polynucleotides

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 912, 435 915, C12Q 168

Patent

active

061596876

ABSTRACT:
A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, whereby
A. extended primers are synthesized by

REFERENCES:
patent: 5023174 (1991-06-01), Ho et al.
patent: 5093257 (1992-03-01), Gray
patent: 5629179 (1997-05-01), Mierendorf
patent: 5830696 (1998-11-01), Short
patent: 5939250 (1999-08-01), Short
patent: 5958672 (1999-09-01), Short
patent: 5965408 (1999-10-01), Short
patent: 6001574 (1999-12-01), Short et al.
patent: 6004788 (1999-12-01), Short
patent: 6030779 (2000-02-01), Short
Marks et al., J. Mol. Biol., vol. 222, pp. 581-597 (1991).
Saiki et al., Reports, pp. 487-491 (Jan. 29, 1988).
Paabo et al., The Journal of Biological Chemistry, vol. 265, No. 8, pp. 4718-4721 (Mar. 15, 1990).
Klug et al., Nucleic Acids Research, vol. 19, No. 10, p. 2793 (May 1991).
Krishnan et al., Nucleic Acids Research, vol. 19, No. 22, pp. 6177-6182.
Gerald F. Joyce, Scientific American, pp. 90-97 (Dec. 1992).
Clackson et al., Nature, vol. 352, pp. 624-628 (Aug. 15, 1991).
Daugherty et al., Nucleic Acids Research, vol. 19, No. 9, pp. 2471-2476.
Yolov et al., Nucleic Acids Research, vol. 18, No. 13, pp. 3983-3986.
Kumud Majumder, Gene, vol. 110, pp. 89-94 (1992).
Jayaraman et al., Proc. Natl. Acad. Sci. USA, vol. 88, pp. 4084-4088 (May 1991).
Mark J. Zoller, Current Opinion in Biotechnology, vol. 3, pp. 348-354 (1992).
Horton et al., BioTechniques, vol. 8, No. 5, pp. 528-535 (1990).
Horton et al., Methods in Enzymology, vol. 217, No. 17, pp. 270-279.
Ho et al., DNA and Protein Engineering Techniques, vol. 2, No. 2, pp. 50-55 (1990).
Dieffenbach et al. Eds. PCR Primer, A Laboratory Manual, p. 521, 1995.
Weber et al., "Formation of Genes Coding For Hybrid Proteins By Recombination Between Related Cloned Genes in E. Coli", Institut For Molekularbiologie I, Universitat Zurich, 8093 Zurich, Switzerland, pp. 5661-5669.
Pompon et al., "Protein Engineering by cDNA Recombination In Yeasts: Shuffling of Mammalian Cytochrome P-450 Functions", 1989 Elsevier Science Publishers B.V., pp. 15-24.
Stemmer et al, "DNA Shuffling By Random Fragmentation And Reassembly In Vitro Recombination For Milecular Evolution", Proc. Natl. Acad. Sci. USA, vol. 91, Oct. 1994, pp. 10747-10751.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for generating recombined polynucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for generating recombined polynucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for generating recombined polynucleotides will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-215340

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.