Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Patent
1998-05-08
2000-12-26
Jones, W. Gary
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
435 6, 536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C12N 1500
Patent
active
06165793&
ABSTRACT:
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
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Jones W. Gary
Kruse Norman
Liebeschuetz Joe
Maxygen Inc.
Whisenant Ethan
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