Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Patent
1996-03-25
2000-09-12
Jones, W. Gary
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
435 6, 536 231, 536 243, 935 76, 935 77, 935 78, C12N 1500, C12Q 168, C07H 2102, C07H 2104
Patent
active
061176796
ABSTRACT:
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4994368 (1991-02-01), Goodman et al.
patent: 5023171 (1991-06-01), Ho et al.
patent: 5043272 (1991-08-01), Hartley
patent: 5093257 (1992-03-01), Gray
patent: 5176995 (1993-01-01), Sninsky et al.
patent: 5187083 (1993-02-01), Mullis
patent: 5223408 (1993-06-01), Goeddel et al.
patent: 5234824 (1993-08-01), Mullis
patent: 5279952 (1994-01-01), Wu
patent: 5316935 (1994-05-01), Arnold et al.
patent: 5356801 (1994-10-01), Rambosek et al.
patent: 5360728 (1994-11-01), Prasher
patent: 5418149 (1995-05-01), Gelfand et al.
patent: 5422266 (1995-06-01), Cromier et al.
patent: 5489523 (1996-02-01), Mathur
patent: 5502167 (1996-03-01), Waldmann et al.
patent: 5521077 (1996-05-01), Khosla et al.
patent: 5541309 (1996-07-01), Prasher
patent: 5556750 (1996-09-01), Modrich et al.
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5605793 (1997-02-01), Stemmer
patent: 5629179 (1997-05-01), Mierendorf et al.
patent: 5652116 (1997-07-01), Grandi et al.
patent: 5679522 (1997-10-01), Modrich et al.
patent: 5714316 (1998-02-01), Weiner et al.
patent: 5723323 (1998-03-01), Kauffman et al.
patent: 5763192 (1998-06-01), Kauffman et al.
patent: 5773267 (1998-06-01), Jacobs
patent: 5783431 (1998-07-01), Peterson et al.
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5814476 (1998-09-01), Kauffman et al.
patent: 5817483 (1998-10-01), Kauffman et al.
patent: 5824485 (1998-10-01), Thompson et al.
patent: 5824514 (1998-10-01), Kauffman et al.
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5834252 (1998-11-01), Stemmer et al.
patent: 5837458 (1998-11-01), Minshull et al.
patent: 5851813 (1998-12-01), Desrosiers
patent: 5858725 (1999-01-01), Crowe et al.
patent: 5939250 (1999-08-01), Short
patent: 5958672 (1999-09-01), Short
patent: 5965408 (1999-10-01), Short
Atreya et al., "Construction of in-frame chimeric plant genes by simplified PCR stragegies," Plant Mol. Biol., 19:517-522 (1992).
Bock et al., "Selection of single-stranded DNA molecules that bind and inhibit human thrombin," Nature, 355:564-566 (Feb. 2, 1992).
Clackson et al., "Making antibody fragments using phage display libraries," Nature, 352:624-628 (Aug. 15, 1991).
Crameri et al., "10(20)-Fold aptamer library amplification without gel purification," Nuc. Acids Res., 21(18):4410 (1993).
Cull et al., "Screening for receptor ligands using large libraries of peptides linked to the C terminus of the lac repressor," PNAS, 89:1865-1869 (Mar. 1992).
Cwirla et al., "Peptides on phage: A vast library of peptides for identifying ligands," PNAS, 87:6378-6382 (Aug. 1990).
Daugherty et al., "Polymerase chain reaction facilitates the cloning, CDR-grafting, and rapid expression of a murine monoclonal antibody directed against the CD18 component of leukocyte integrins," Nuc. Acids Res., 19(9):2471-2476 (1991).
Delagrave et al., "Searching Sequence Space to Engineer Proteins: Exponential Ensemble Mutagenesis," Biotechnology, 11:1548-1552 (Dec. 1993).
Dube et al., "Artificial mutants Generated by the Insertion of Random Oligonucleotides into the Putative Nucleoside Binding Site of the HSV-1 Thymidine Kinase Gene," Biochemistry, 30(51):11760-11767 (1991).
Ghosh et al., "Arginine-395 Is Required for Efficient in Vivo and in Vitro Aminoacylation of tRNAs by Escherichia coli Methionyl-tRNA Stnthetase," Biochemistry, 30:11767-11774 (1991).
Goldman et al., "An Algorithmically Optimized Combinatorial Library Screened by digital Imaging Spectroscopy," Biotechnology, 10:1557-1561 (Dec. 1992).
Harlow et al., "Construction of Linker-Scanning Mutations using the Polymerase Chain Reaction," Methods in Mol. Biol., 31:87-96 (1994).
Heda et al., "A simple in vitro site directed mutagenesis of concatamerized cDNA by inverse polymerase chain reaction," Nuc. Acids Res., 20(19):5241-5242 (1992).
Ho et al., "DNA and Protein Engineering Using the Polymerase Chain Reaction: Splicing by Overlap Extension," DNA and Protein Eng. Techniques, 2(2):50-55 (1990).
Hodgson, "The Whys and Wherefores of DNA Amplification," Biotechnology, 11:940-942 (Aug. 1993).
Horton et al., "Gene Splicing by Overlap Extension," Mehtods in Enzymology, 217:270-279 (1993).
Horton et al., "Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase chain Reaction," BioTechniques, 8(5):528-535 (May 1990).
Jayaraman et al., "Polymerase chain reaction-mediated gene synthesis: Synthesis of a gene coding for isozyme c of horseradish peroxidase," PNAS, 88:4084-4088 (May 1991).
Jones et al., "A Rapid Method for Recombination and Site-Specific Mutagenesis by Placing Homologous ends on DNA Using Polymerase Chain Reaction," BioTechniques, 10(1): 62-66 (1991).
Joyce, G. F., "Directed Molecular Evolution," Scientific American, (Dec. 1992).
Klug et al., "Creating chimeric molecules by PCR directed homologous DNA recombination," Nuc. Acids Res., 19(10):2793 (1991).
Krishnan et al., "Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of .lambda. phage clones," Nuc. Acids Res., 19(22):6177-6182 (1991).
Majumder, K., "Ligation-free gene synthesis by PCR: synthesis and mutagenesis at multiple loci of a chimeric gene encoding OmpA signal peptide and hirudin," Gene, 110:89-94 (1992).
Marks et al., "By-passing Immunization, Human Antibodies from V-gene Libraries Displayed on Phage," J. Mol. Biol., 222:581-597 (1991).
McCafferty et al., "Phage antibodies: filamentous phage displaying antibody variable domains," Nature, 348:552-554 (Dec. 6, 1990).
Morl et al., "Group II intron RNA-catalyzed recombination of RNA in vitro," Nuc. Acids Res., 18(22):6545-6551 (1990).
Mullis et al., "Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction," Methods in Enzymology, 155:335-351 (1987).
Mullis et al., "Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction," Cold Spring Harbor Symposia on Quantitative Biology, 51:263-273 (1986).
Nissim et al., "Antibody fragments from a `single pot` display library is immunochemical reagents," EMBO Journal, 13(3):692-698 (1994).
Osuna et al., "Combinatorial mutagenesis of three major groove-contacting residues of Eco RI: single and double amino acid replacements retaining methyltransferase-sensitive activities," Gene, 106:7-12 (1991).
Paabo et al., "DNA Damage Promotes Jumping between Templates during Enzymatic Amplification," J. Biol. Chem., 265(8):4718-4721 (Mar. 15, 1990).
Saiki et al., "Diagnosis of sickle Cell Anemia and .beta.-Thalassemia with Enzymatically Amplified DNA and Nonradioactive Allele-Specific Oligonucleotide Probes," New England J. of Medicine, 319(9):537-541 (Sep. 1, 1988).
Saiki et al., "analysis of enzymatically amplified .beta.-globin and HLA-DQ.alpha. DNA with allele-specific oligonucleotide probes," Nature, 324:163-166 (Nov. 13, 1986).
Saiki et al., "Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site analysis for Diagnosis of Sickle Cell Anemia," Science, 230:1350-1354 (Dec. 20, 1985).
Saiki et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostabl;e DNA Polymerase," Science, 239:487-491 (Jan. 20, 1988).
Sambrook et al., Molecular Cloning, A Laboratory Manual, Cold Spring Laboratory Press, Cold Spring Harbor, New York (1989).
Scharf et al., "Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences," Science, 233:1076-1078 (Sep. 1986).
Scott et al., "Searching for Peptide Ligands with an Epitope Library," Science, 249:386-390 (Jul. 20, 1990).
Sikorski et al., "In Vitro Mutagenesis and Planned Shuffling: From
Jones W. Gary
Kruse Norman J.
Liebeschuetz Joe
Maxygen Inc.
Whisenant Ethan
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