Methods for generating polynucleotides having desired...

Details Methods for generating polynucleotides having desired... Methods for generating polynucleotides having desired...

2011-07-19

2011-07-19

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S440000, C536S023100

Reexamination Certificate

active

07981614

ABSTRACT:
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

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