Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence
Patent
1991-06-05
1993-09-28
Wityshyn, Michael G.
Chemistry: analytical and immunological testing
Optical result
With fluorescence or luminescence
435 772, 435 19, 435 21, 549332, 549510, G01N 2176, G01N 3353, C12Q 144, C07D30300
Patent
active
052486184
ABSTRACT:
Novel assay methods employing compounds which are chemically or enzymatically cleavable and which give rise to an intermediate which further decomposes by an intramolecular anchimeric displacement reaction which releases a signal producing species are disclosed. Also disclosed are probe hybridization assays employing the compounds of the invention employing thermostable enzymes which are not denatured by the hybridization conditions. Such signal producing species may include chemiluminescent dioxetanes and other colored products.
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Gitomer Ralph
Life Technologies Inc.
Wityshyn Michael G.
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