Methods for generating cytotoxic T cells in vitro

Details Methods for generating cytotoxic T cells in vitro Methods for generating cytotoxic T cells in vitro

1996-10-07

2000-10-10

Cunningham, Thomas M.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

435375, 435377, C12N 506, C12N 508

Patent

active

06130087&

ABSTRACT:
The present invention provides methods for generating antigen-reactive cytotoxic T cells in vitro comprising culturing immune cells and antigenic cells that have at least one MHC allele in common (and preferably, are syngeneic), in which the antigenic cells have been treated according to the methods of the invention. The antigenic cells are treated by subjecting them to osmotic shock followed by irradiation. As a result, a subset of T cells are activated and mature into antigen-reactive cytotoxic T cells. The effectiveness of the procedure may be enhanced by repeated restimulations and/or the addition of heat shock protein-peptide complexes. Methods and compositions are also disclosed for the treatment and prevention in a subject of cancer or infectious disease comprising administering to the subject matched cytotoxic T cells that are generated in vitro by the present methods.

REFERENCES:
patent: 4690915 (1987-09-01), Rosenberg
Engers et al. S. of Immunology 115:356-360, 1975.
Umezu et al. Cancer Immunol. Immunother. 37:392-397. 1993.
Nair et al. J. of Imm. Methods 152:232. 1992.
Anichini et al., 1987, Immunol. Today 8:385-389.
Barrios, 1992, Eur. J. Immunol. 22:1365-1372.
Bartlett, 1972, J. Natl. Cancer Inst. 49:493-504.
Berke, 1995, Cell 81:9-12.
Bevan, 1995, J. Exp. Med. 182:639-641.
Blachere and Srivastava, 1993, J. Cell. Biochem. Keystone Symp. NZ502, p. 124.
Blachere er al., 1993, J. Immunother. 14:352-356.
Cohen, 1987, Basic and Clinical Immunology (Appleton & Lange, Norwalk) pp. 669-689.
Falk et al., 1990, Nature 348:248-251.
Falk et al., 1991, Nature 351:290-296.
Fathman et al., 1989, Fundamental Immunology (Raven Press Ltd., New York) pp. 803-815.
Feldweg and Srivastava, 1993, J. Cell. Biochem. Suppl., yAbstract NZ 108.
Fossati et al., 1982, Cancer Immunol. Immunother. 14:99-104.
Gartner et al., 1986, Science 233:215-217.
Greenberg et al., 1983, Basic and Clinical Tumor Immunology (Martinus Nijhoff Publishers, Boston) pp. 301-335.
Grimm et al., 1982, J. Exp. Med. 155:1823-1841.
Herin et al., 1987, Int. J. Cancer 39:390-396.
Hersey et al., 1981, Int. J. Cancer 28:695-703.
Lakey et al., 1997, Proc. Natl. Acad. Sci. USA 84:1659-1663.
Lanzavecchia, 1993, Curr. Opin. Immunol. 8:348-354.
Lanzavecchia, 1993, Science 260:937-944.
Levy, 1991, Cell 67:265-274.
Li and Srivastava, 1993, EMBO J. 12:3143-3151.
Lindquist and Craig, 1988, Ann. Rev. Genet. 22:631-677.
Luescher et al., 1991, Nature 351:72-77.
Lussow et al., 1991, Eur. J. Immunol. 21:2297-2302.
Melief and Kast, 1992, Cancer Surveys Vol. 13: A New Look at Tumor Immunology, Imperial Cancer Research Fund pp. 81-99.
Mule et al., 1984, Science 225:1487-1489.
Old et al., 1962, Ann. N.Y. Acad. Sci. 101:80-106.
Palladino et al., 1987, Cancer Res. 47:5074-5079.
Rosenberg et al., 1988, New Engl. J. Med. 316:889-897.
Rosenberg et al., 1988, New Engl. J. Med. 319:1676-1680.
Rotzschke et al., 1990, Nature 348:248-251.
Rudensky et al., 1991, Nature 353:622-627.
Sallusto and Lanzavecchia, 1994, J. Exp. Med. 179:1109-1118.
Schumacher et al., 1991, Nature 350:703-706.
Slovin et al., 1986, J. Immunol. 9:3042-3048.
Srivastava et al., 1994, Immunogenetics 39:93-98.
Srivastava et al., 1987, Proc. Natl. Acad. Sci. USA 84:3807-3811.
Srivastava and Maki, 1991, Microbiol. Immunol. 167:109-123.
Srivastava and Heike, 1986, Sem. in Immunol. 3:57-64.
Srivastava et al., 1989, Cancer Res. 49:1341-1343.
Srivastava et al., 1986, Proc. Natl Acad. Sci. USA 83:3407-3411.
Srivastava et al., 1991, Curr. Opin. Immunol. 3:654-658.
Srivastava et al., 1984, Int. J. Cancer 33:417-422.
Srivastava et al., 1988, Immunol. Today 9:78-83.
Suto and Srivastava, 1995, Science 269:1585-1588.
Taylor and Cohen, 1992, Curr. Opin. Immunol. 4:338-343.
Topallian et al., 1989, J. Immunol. 142:3714-3725.
Udono et al., 1993, J. Exp. Med. 178:1391-1396.
Udono, 1993, J. Cell. Biochem. Suppl. 17D:113 (Abs. NZ225).
Ullrich et al., 1986, Proc. Natl. Acad. Sci. USA 83:3121-3125.
Valitutti et al., 1995, Nature 375:148-151.
Vanbuskirk et al., 1989, J. Exp. Med. 170:1799-1809.
Yewdell and Bennink, 1992, Adv. Immunol. 52:1-123.
Young, 1990, Ann. Rev. Immunol. 8:401-420.
Zarling et al., 1978, Nature 274:269-271.
Barrios et al., 1994, "Specificity of antibodies induced after immunization of mice with the mycobacterial heat shock protein of 65kD", Clin. Exp. Immunol., 98:224-228.
Barrios et al., 1994, "Heat shock protein as carrier molecules: in vivo helper effect mediated by Escherichia coli GroEL and DnaK proteins requires cross-linking with antigen", Clin. Exp. Immunol., 98:229-233.
Moore et al., 1988 "Introduction of soluble protein into the Class I pathway of Antigen processing and presentation", Cell 54:777-785.

Affiliated with

Also associated with

Rating

Methods for generating cytotoxic T cells in vitro does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for generating cytotoxic T cells in vitro, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for generating cytotoxic T cells in vitro will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2255449