Methods for generating cellulases

Details Methods for generating cellulases Methods for generating cellulases

2008-09-09

2008-09-09

Mondesi, Robert B. (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C530S350000, C536S023100

Reexamination Certificate

active

11034411

ABSTRACT:
The invention relates to carboxymethyl cellulases and to polynucleotides encoding the carboxymethyl cellulases. In addition methods of designing new carboxymethyl cellulases and method of use thereof are also provided. The carboxymethyl cellulases have increased activity and stability at increased pH and temperature.

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Bronnenmeier et al. Purification of Thermotoga maritima enzymes for degradation of cellulose material. Appl. Environ. Microbiol. 61: 1399-1407, Apr. 1995.
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Gilbert et al. Evidence for multiple of carboxymethylcellulase genes inPseudomonas fluorescenssubsp. cellulosa. Mol. Gen. Genet. 210: 551-556, 1987.
Shima, S. et al. Nucleotide sequence analysis of the endoglucanase encoding gene, celCCD, of Clostridium cellulolyticum. Gene 104(1):33-8, Jul. 31, 1991.

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