Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-04-24
1998-10-20
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 4351723, 4353201, 536 231, C12Q 168, C12P 2102, C12N 1564, C07H 2102
Patent
active
058244859
ABSTRACT:
The present invention relates to a novel drug discovery system for generating and screening molecular diversity. The system provides methods for mixing and cloning genetic materials from a plurality of species of organisms in combinatorial gene expression libraries to generate novel metabolic pathways and classes of compounds. The system also involves methods for pre-screening or identifying for host organisms containing a library that are capable of generating such novel pathways and compounds. The host organisms may be useful in drug screening for particular diseases, and in commercial production of compounds of interest. The methods of the invention are also useful in preserving the genomes of organisms that are known or prospective sources of drugs.
REFERENCES:
patent: 4399219 (1983-08-01), Weaver
patent: 5326691 (1994-07-01), Hozier
patent: 5434065 (1995-07-01), Mahan et al.
patent: 5441885 (1995-08-01), Goldberg
patent: 5506126 (1996-04-01), Seed et al.
patent: 5527896 (1996-06-01), Wigler et al.
Arisawa et al., "Cloning of the Macrolide Antibiotic Biosynthesis Gene AcyA, Which Encodes 3-0-Acyltransferase, From Streptomyces Thermotolerans and Its Use Direct Fermentative Production of a Hybrid Macrolide Antibiotic", Appl. Env. Micro. 60(7):2657-2660 (1994).
Fu et al., "Engineered Biosynthesis of Novel Polyketides: Stereochemical Course of Two Reactions Catalyzed by a Polyketide Synthase" Biochemistry 33:9321-9326 (1994).
Kao et al., "Engineered Biosynthesis of a Complete Macrolactone in a Heterologous Host" Science 265:509-511 (1994).
Rani et al., (1994), "Cloning and Expression of the Mercury Resistance Genes of Marine Pseudomonas Sp. Strain MR1 Plasmid pMR1 in Escherichia Coli," Res Microbiol, 145:121-127.
McDaniel et al., "Engineered Biosynthesis of Novel Polyketides", Science 262:1546-1550 (1993).
Murdock et al., "Construction of Metabolic Operon Catalyzing the De Novo Biosynthesis of Indigo in Escherichia Coli" Bio/Technology 11:381-385 (1993).
Steglitz-Morsdorf et al., (1993), "Cloning Heterologous Expression and Sequencing of the Proteus Vulgaris glnAntrBC Operon, and Implications of Nitrogen Control on Heterologous Urease Expression", FEMS Microbiol Lett, 106:157-164.
LaCalle et al., "Cloning of the Complete Biosynthetic Gene Cluster for an Aminonucleoside Antibiotic, Puromycin, and its Regulated Expression in Heterologous Hosts" EMBO J. 2:785-792 (1992).
Hundle et al., "Cartenoids of Erwinia Herbicola and an Escherichia Coli HB101 Strain Carrying the Erwinia Herbicola Gene Cluster", Photochem. and Photobiol. 54(1):89-93 (1991).
Thanabalu et al., "Cloning, Sequencing and Expression of a Gene Encoding a 100-Kilodalton Mosquitocidal Toxin from Bacillus sphaericus SSII-1" J. Bact. 173:2776-2785 (1991).
Sarwar et al., "Cloning of Aminoglycoside Phosphotransferase (APH) Gene from Antbiotic-Producing Strain of Bacillus Circulans into a High-Expression Vector, pKK233-3", Biochem. J. 268:671-677 (1990).
Rojo et al., "Assemblage of Ortho Cleavage Route for Simultaneous Degradation of Chloro- and Methylaromatics" Science 238:1395 (1987).
Malpartida et al., "Molecular Cloning of the Whole Biosynthetic Pathway of a Streptomyces Antibiotic and its Expression in a Heterologous Host", Nature 309:462-464 (1984).
Ensley et al., "Expression of Napthalene Oxidation Genes in Escherichia Coli Results in the Biosynthesis of Indigo" Science 222:167-169 (1983).
Dunlap et al., "Characterization of a Periplasmic 3':5'-Cyclic Nucleotide Phosphodiesterase Gene, cpdP, from the Marine Symbiotic Bacterium Vibrio Fischeri", J. Bact. 175(15):4615-4624 (1993).
Fukagawa et al., "Cloning of Gene Responsible for Tributyltin Chloride (TBTCI) Resistance in TBTCI-Resistant Marine Bacterium, Alteromanas S. M-1", Biochem. Biophys. Res. Comm. 194(2):733-740 (1993).
Tsujibo et al., "Cloning, Sequence, and Expression of a Chitinase Gene from a Marine Bacterium, Alteromonas Sp. Strain 0-7", J. Bact. 175(1):176-181 (1993).
Danaher et al., "Expression of Cloned Restriction and Modification Genes, hjaIRM from Hyphomanas Jannaschiana in Escherichia Coli", Gene 89:129-133 (1992).
David et al., "Cloning, Sequencing, and Expression of the Gene Encoding the Extracellullar Neutral Protease, Vibriolysin of Vibrio Proteolyticus", Gene 112:107-112 (1992).
MacLeod et al., "Identification and Sequence of Na+-Linked Gene from the Marine Bacterium Alteromonas Haloplanktis Which Functionally Complements the dagA Gene of Escherichia Coli", Mol Micro. 6(18):2673-2681 (1992).
Sorum et al., "Identification and Cloning of a Tetracycline Resistance Gene From the Fish Pathogen Vibrio Salmonicida", Antimicro. Agents Chemo. 36(3):611-615 (1992).
Fuqua et al., "Characterization of melA: A Gene Encoding Melanin Biosynthesis from the Marine Bacterium Shewanella Colwelliana", Gene 109:131-136 (1991).
Nakamura et al., "Cloning and Sequencing of an Na+/H + Antiporter Gene from the Marine Bacterium Vibrio Alginolyticus"., Biochim. Biophys. Acta 1190:465-468 (1990).
Barbeyron et al., "The Gene Encoding the Kappa-Carrageenase of Alteromonas Carrageenovora is Related to Beta-1,3-1, 4-Glucanases"., Gene 139:105-109 (1982).
Huse, "Construction of Combinatorial Antibody Expression Libraries in Escherichia Coli" CIBA Foundation Symposium 159, pp. 91-102 (1991).
Simonsen et al., "Cloning by Function: Expression Cloning in Mammalian Cells" Trends in Pharm. Sci. 15:437-441 (1994).
Luyten et al., "Receptor Cloning and Heterologous Expression Towards a New Tool for Drug Discovery" Trends in Biotech. 11:247-54 (1993).
Nakayama et al., "Use of Mammalian Cell Expression Cloning Systems to Identify Genes for Cytokines, Receptors and Regulatory Proteins" Curr. Opin. Biotech. 3:497-505 (1992).
Shizuya et al., "Cloning and Stable Maintenance of 300-Kilobase-Pair Fragments of Human DNA in E. Coli Using an F-Factor-Based Vector", Proc. Natl. Acad. Sci. 89:8794-8797 (1992).
Aruffo, "Expression Cloning Systems" Curr. Opin. Biotech. 2:735-741 (1991).
Clarke et al., "A Colony Bank Containing Synthetic Col E-1 Hybrid Plasmids Representative of the Entire E. Coli Genome", Cell 9:91-99 (1976).
Hutchinson, "Drug Synthesis by Genetically Engineered Microorganisms" Bio Technology 12:375-379 (1994).
Ecker et al., "Combinatorial Drug Discovery: Which Methods Will Produce the Greatest Value?," Bio/Technology 13:351-360 (Apr. 1995).
Katz et al., "Polyketide Synthesis Prospects for Hybrid Antibioitics", Am. Rev. Microbiol. 47:875-912 (1993).
Fenical and Jenson, Marine Microorganisms: A New Biomedical Resource. Advances in Marine Biotechnology, vol. I: Pharmaceutical and Bioactive Natural Products, pp. 419-457, D. Attaway, O. Zaborsky eds., Plenum Press, New York. (1992).
Hopwood et al., in Secondary Metabolites: Their function and Evolution, Wiley, Clichester (CIBA Foundation symposium 171)pp. 88-122 (1992).
Floss et al., "Genetic Engineering of Hybrid Antibiotics-a Progress Report", Tetrahedron 47:6045-6058 (1991).
Tapiolas et al., "Octalactins A and B, Cytotoxic 8-Membered Ring Lactones from the Marine Bacterium Streptomyces Sp.", J. Amer. Chem. Soc. 113:4682-4683 (1991).
Orser et al., "Use of Prokaryotic Stress Promoters as Indicators of the Mechanism of Chemical Toxicity", In Vitro Toxicology 8(1):71-85 (1995).
Cohen et al., "Genetic and Functional Analysis of the Multiple Antiobiotic Resistance (MAR) Locus in Escherichia Coli", J. Bact. 175(5):1484-1492 (1993).
Cohen et al., "Salicylate Induction of Antibiotic Resistance in Escherichia Coli: Activation of the MAR Operon and a MAR-Independent Pathway", J. Bact. 175(24):7859-7862 (1993).
Corbisier et al., "LuxAB Gene Fusions with the Arsenic and Cadmium Resistance Operons of Staphylococcus Aureus Plasmid p1258", FEMS Microbiology Letters 110(2):231-238 (1993).
de Lorenzo et al., "Engineering of Alkyl-and Haloaromatic-Responsive Gene Expression with Mini-Transposons Containing Regulated Promoters of Biodegradative Pathways of Pseudomonas", Gene 130:41-46 (1993).
Metcalf et al., "Mutational Analysis of an Escherichia Coli Fourteen-Gene Operon for Phosphonate Degradation, Using TnphoA' Elements", J. Bact. 175(11):3430-3442 (1
Brian Paul
Foster Lyndon M.
Nasby Nicole Marie
Peterson Todd C.
Thompson Katie A.
Brusca John S.
Chromaxome Corporation
Ketter James
LandOfFree
Methods for generating and screening novel metabolic pathways does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for generating and screening novel metabolic pathways, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for generating and screening novel metabolic pathways will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-242945