Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-01-03
1998-11-17
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435243, 435259, 424 91, 252351, C12Q 168, C12N 100, C12N 106, B01F 1700
Patent
active
058374523
ABSTRACT:
Methods for extracting nucleic acids by heating sample cells at about 80-95 degrees C. in a permeabilization reagent containing a non-ionic detergent and a metal chelating agent. The methods of the invention release large fragments of undegraded nucleic acids without physically disrupting the entire cell wall. The nucleic acids are released into solution without bursting the cells and are suitable for research and testing without further purification. The extraction method described herein is rapid, easy to perform, and applicable to a wide variety of cells, including microorganisms. Clinical samples may be screened for the presence or absence of a microorganism by heating the sample at 80-95 degrees Celsius in the presence of a non-ionic detergent and a metal chelating agent, adding to the sample a nucleic acid probe specific to the selected microorganism, incubating the sample under conditions which allow the probe to hybridize to released nucleic acid and detecting whether any hybridized probe is present. A kit for performing the nucleic acid extraction methods disclosed herein is also described.
REFERENCES:
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4830969 (1989-05-01), Holmes
patent: 5212059 (1993-05-01), Schwartz et al.
patent: 5231015 (1993-07-01), Cummins et al.
Derwent Publication Ltd London GB, Data Base WPI, Abstract. Week DW9303, JP4349892 (1992).
H. Meijer, et al., "Recovery and Identification of DNA Sequences Harboured In Preserved Ancient Human Bones", Biochem. Biosphys. Res. Commun., 183 : (2) :367-374, (Mar. 16, 1992).
Mifflin et al, "Use and application of nucleic acid probes in the clinical laboratory", Clin. Chem. 35(9):1819-1825, 1989.
Doi et al., "Isolation of nuclei from a tetraploid strain of saccharomyces cerevisiae", J. Biochem. 75:1017-1026, 1974.
Ulenhopp, et al., Viscoelastic Charactization of Single-Stranded DNA. Biophys. J. 15:223-232 (1975).
Godson, et al., Lysis of E. Coli with a neutral detergent. Biochem. Biophys. Acta 149:476-088 (1967).
Lindblom et al, (1988), "Rapid DNA purification for restriction fragment length polymurphism analysis", Gene Anal. Techn. 5:97-101.
Ortlepp et al, (1989), "An Improved boiling method for the preparation of bacterial plasmid and phage DNA", Gene Anal. Techn. 6:96-96.
Arnold et al, (1989), "Assay formats involving acridinium ester labeled probes", Clin. Chem. 35(8):1588-1594.
Stratigene Catalog, (1988), "Gene characterization kits", p. 39.
Darnell et al. (1986), "RNA synthesis and processing in eukaryotes", in Molecular Cell Biology, Scientific American Books, New York, pp. 322-323.
New England Biolabs catalog, (1992), p. 73.
Sritharan et al, (1991) "A simple method for diagnosising M. tuberculosis infection in clinical samples using PCR", Mol. Cell. Probes 5:385-395.
Clark Kathleen A.
Kacian Daniel L.
Cappellari Charles B.
Fisher Carlos A.
Fredman Jeffrey
Gen-Probe Incorporated
Jones W. Gary
LandOfFree
Methods for extracting nucleic acids from a wide range of organi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for extracting nucleic acids from a wide range of organi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for extracting nucleic acids from a wide range of organi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-882861