Methods for extracting and purifying kallidinogenase

Details Methods for extracting and purifying kallidinogenase Methods for extracting and purifying kallidinogenase

1976-04-07

1977-07-26

Shapiro, Lionel M.

Textiles: fiber preparation

C07G 7026, C12D 1310

Patent

active

040381412

ABSTRACT:
A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.

REFERENCES:
patent: 3809748 (1974-05-01), Khouw et al.
patent: 3905870 (1975-09-01), Kutzbach et al.

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