Methods for enriching target nucleic acid sequences

Details Methods for enriching target nucleic acid sequences Methods for enriching target nucleic acid sequences

1996-03-29

1998-06-02

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

057597804

ABSTRACT:
The invention includes selective amplification methods for enriching target nucleic acid sequences that are present in a first source of nucleic acid but absent from a second source of nucleic acid. These methods involve using phenol to enhance the rate of the hybridization step, as well as modifications for reducing the amounts of reagents required for the reactions. Also included are methods for identifying sequences shared by two or more populations of nucleic acids.

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