Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-08-31
1997-07-08
Green, Lora M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
210781, 210782, 435 2, 435 721, 435 724, 435803, 436514, 436518, 436527, 436824, 422 72, 422101, 422102, G01N 33555, B01L 1100
Patent
active
056460040
ABSTRACT:
The present invention relates to methods of enriching fetal cells from maternal body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a gradient solution adjusted to a specific density to enrich for fetal nucleated red blood cells from maternal blood. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to undesired cell populations allowing the fetal cells to be separated during centrifugation in a more convenient manner. The rapid fetal cell enrichment method described herein has a wide range of applications, including but not limited to, gender determination and prenatal diagnosis of genetic diseases without the use of invasive procedures.
REFERENCES:
patent: 3441205 (1969-04-01), Young, Jr.
patent: 3513976 (1970-05-01), James
patent: 3706305 (1972-12-01), Berger et al.
patent: 3706306 (1972-12-01), Berger et al.
patent: 3750645 (1973-08-01), Bennett et al.
patent: 3849072 (1974-11-01), Ayres
patent: 3862303 (1975-01-01), Anderson
patent: 3937211 (1976-02-01), Merten
patent: 3957654 (1976-05-01), Ayres
patent: 3957741 (1976-05-01), Rembaum et al.
patent: 3965889 (1976-06-01), Sachs
patent: 3985122 (1976-10-01), Topham
patent: 4001122 (1977-01-01), Griffin
patent: 4020831 (1977-05-01), Adler
patent: 4022576 (1977-05-01), Parker
patent: 4035316 (1977-07-01), Yen et al.
patent: 4040959 (1977-08-01), Berman et al.
patent: 4055501 (1977-10-01), Cornell
patent: 4066414 (1978-01-01), Selby
patent: 4105598 (1978-08-01), Yen et al.
patent: 4112924 (1978-09-01), Ferrara et al.
patent: 4134512 (1979-01-01), Nugent
patent: 4147628 (1979-04-01), Bennett et al.
patent: 4152270 (1979-05-01), Cornell
patent: 4181700 (1980-01-01), Chervenka et al.
patent: 4203840 (1980-05-01), Stoeppler
patent: 4213456 (1980-07-01), Bottger
patent: 4256120 (1981-03-01), Finley
patent: 4378812 (1983-04-01), Sarstedt
patent: 4443345 (1984-04-01), Wells
patent: 4459997 (1984-07-01), Sarstedt
patent: 4510244 (1985-04-01), Parks et al.
patent: 4511349 (1985-04-01), Nielsen et al.
patent: 4511662 (1985-04-01), Baran et al.
patent: 4533468 (1985-08-01), Ensor et al.
patent: 4562844 (1986-01-01), Carpenter et al.
patent: 4569764 (1986-02-01), Satchell
patent: 4610846 (1986-09-01), Martin
patent: 4707276 (1987-11-01), Dodge et al.
patent: 4710472 (1987-12-01), Saur et al.
patent: 4777145 (1988-10-01), Luotola et al.
patent: 4824560 (1989-04-01), Alspector
patent: 4828716 (1989-05-01), McEwen et al.
patent: 4844818 (1989-07-01), Smith
patent: 4886071 (1989-12-01), Mehl et al.
patent: 4917801 (1990-04-01), Luderer et al.
patent: 4927749 (1990-05-01), Dorn
patent: 4927750 (1990-05-01), Dorn
patent: 4954264 (1990-09-01), Smith
patent: 4957638 (1990-09-01), Smith
patent: 4983369 (1991-01-01), Barder et al.
patent: 5030341 (1991-07-01), McEwen et al.
patent: 5030559 (1991-07-01), Nicolson et al.
patent: 5039401 (1991-08-01), Columbus et al.
patent: 5045201 (1991-09-01), Dubois et al.
patent: 5053134 (1991-10-01), Luderer et al.
patent: 5132232 (1992-07-01), Parker
patent: 5236604 (1993-08-01), Fiehler
patent: 5248480 (1993-09-01), Greenfield et al.
patent: 5260186 (1993-11-01), Cercek et al.
patent: 5269927 (1993-12-01), Fiehler
patent: 5271852 (1993-12-01), Luoma, II
patent: 5279936 (1994-01-01), Vorpahl
patent: 5308506 (1994-05-01), McEwen
patent: 5314074 (1994-05-01), Inbar
patent: 5474687 (1995-12-01), Van Vlasselaer
Bianchi et al., "Isolation of fetal DNA from nucleated erythrocytes in maternal blood," Proc. Nat. Acad. Sci., vol. 87, pp. 3279-3283 (1990).
Wallach et al., "Affinity Density Perturbation: A New Fractionation Principle and Its Illustration in a Membrane Separation," FEBS Letters, vol. 21, No. 1, pp. 29-33, (1972).
"The CD System," Dako, Inc. (1990).
Herzenberg et al., 1979, "Fetal Cells in the Blood of Pregnant Women: Detection and Enrichment by Fluorescence-Activated Cell Sorting" Proc. Natl. Aca. Sci, USA 76: 1453-5.
Price et al., 1991, "Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry," Am. J. Obstet. Gynecol. 165(6), part 1):1731-1737.
Elias et al., 1992, Session 12: Plenary Session, "Prenatal diagnosis of aneuploidy using fetal cells isolated from maternal blood" Am. J. Hum. Genet, 51:A4, Excerpt 5.
Ganshirt-Ahlert et al., 1992, Session 34: Prenatal and Perinatal Genetics II, and Molecular Applications in Clinical Genetics III, "Noninvasive prenatal diagnosis," Am. J. Hum. Genet. 51:A48 Excerpt 182.
Harrison et al., 1992, Prenatal and Perinatal Genetics, "Use of fluorescence in situ hybridization to detect confined placental mosaicism in trisomic conceptions," Am. J. Hum. Genet. 51:A257, Excerpt 1014.
Holzgreve et al., 1992, "Fetal Cells in the Maternal Circulation," J. Reprod. Med. 37(5):410-418.
Julien et al., 1992, Session 34: Prenatal and Perinatal Genetics II, and Molecular Applications in Clinical Genetics III, "Fetal cells in maternal blood," Am. J. Hum. Genet. 51:A48, Excerpt 181.
Russo et al., 1992, The Use of Resealed Erythrocytes as Carrier's and Bioreactors, Published by Magnani & DeLoach, Plenun Press, New York, pp. 101-107.
Dicke et al., 1968, "The Selective Elimination of Immunologically Competent Cells From Bone Marrow and Lymphatic Cell Mixtures," Transplantation 6(4):562-570.
Dicke et al., 1970, "Avoidance of Acute Secondary Disease by Purification of Hemopoietic Stem Cells with Density Gradient Centrifugation," Exp. Hematol. 20:126-130.
Dicke et al., 1971, "Allogeneic Bone Marrow Transplantation After Elimination of Immunocompetent Cells by Means of Density Gradient Centrifugation," Transplantation Proceedings 3(1):666-668.
Dicke et al., 1973, "The Use of Stem Cell Concentrates As Bone Marrow Grafts in Man," Transplantation Proceedings 5(1):909-912.
Korbling et al., 1977, "Procurement of Human Blood Stem Cells by Continuous-Flow Centrifugation --Further Comment," Blood 50:753-754.
Korbling et al., 1977, "In-Vitro and In-Vivo Properties of Canine Blood Mononuclear Leukocytes Separated by Discontinuous Albumin Density Gradient Centrifugation," Biomedicine, 26:275-283.
Olofsson et al., 1980, "Separation of Human Bone Marrow Cells in Density Gradients of Polyvinylpyrrolio Coated Silica Gel (Percoll)," Scand. J. Hemattol. 24:254-262.
Osborne et al., 1980, "The Value of Estrogen and Progesterone Receptorsin the Treament of Breast Cancer" Cancer 46(12):2884-8.
Westley et al., 1980, "An Estrogen-Induced Protein Secreted By Human-Breast Cancer Cells in Culture" European Journal of Cell Biology 22(1):397.
Gerdes et al., 1983 "Production of a Mouse Monoclonal Antibody Reactive with a Human Nuclear" Current Biotech Abs.
Ellis et al., 1984, "The Use of Discontinuous Percoll Gradients to Separate Populations of Cells from Human Bone Marrow and Peripheral Blood," J. of Immunological Methods 66:9-16.
Kufe et al., 1984, "Differential Reactivity of a Novel Monoclonal Antibody (DF3) with Human Malignant Versus Benign Breast Tumors" Hybridoma 3(3):223-32.
Lasky et al., 1985, "Size and Density Characterization of Human Committed and Multipotent Hematopoietic Progenitors" Exp. Hematol. 13:680-4.
Martin et al., 1986, "Purification of Haemopoietic Progenitor Cells From Patients with Chronic Granulocytic Leukaemia Using Percoll Density Gradients and Elutriation" Brit. J. Haematol. 63:187-98.
Bray et al., 1987 "Serum Levels and Biochemical Characteristics of Cancer-Associated Antigen CA-549, a Circulating Breast Cancer Marker" Cancer Res 47(22):5853-60.
Shpall et al., 1991, "Immunomagnetic purging of breast cancer from bone marrow for autologous transplantation," Bone Marrow Transplantation 7:145-151.
Yoshioka et al., 1991, "Immobilization of ultra-thin layer of monoclonal antibody on glass surface," J. of Chromolography 566:361-368.
Durrant et al., 1992 "A Rapid Method for Separating Tumour Infiltrating Cells and
Activated Cell Therapy, Inc.
Dehlinger Peter J.
Glaister Debra J.
Green Lora M.
Stratford Carol A.
LandOfFree
Methods for enriching fetal cells from maternal body fluids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for enriching fetal cells from maternal body fluids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for enriching fetal cells from maternal body fluids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2408281