Methods for enhancing the production of viral vaccines in PKR-de

Details Methods for enhancing the production of viral vaccines in PKR-de Methods for enhancing the production of viral vaccines in PKR-de

1996-08-21

1998-11-24

Stucker, Jeffrey

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435 5, 435 692, 435239, 4242061, 4242041, 530350, 536 232, 536 245, 536 252, 536 253, C12N 700, A61K 3912, C07H 2100, C12Q 170

Patent

active

058405658

ABSTRACT:
Methods for enhancing the production of viral vaccines in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain interferon induced antiviral activities, in particular, cellular levels of double-stranded RNA (dsRNA) dependent kinase (PKR), PKR-deficient cells are obtained by a) transfecting a parent cell line with a PKR antisense polynucleotide; b) unaided uptake into a cell line by culturing said cell line in the presence of a PKR antisense polynucleotide; c) transfection of a parent cell line with a PKR dominant negative mutant; or d) culturing a cell line in the presence of 2-aminopurine. In cell cultures deficient for PKR, viral yield is enhanced by several orders of magnitude over cell cultures with normal levels of these proteins making these cell cultures useful for the production of viral vaccines.

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