Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2007-10-30
2007-10-30
Kruse, David H (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C435S320100, C435S468000
Reexamination Certificate
active
10190217
ABSTRACT:
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
REFERENCES:
patent: 5731179 (1998-03-01), Komari et al.
patent: 6521458 (2003-02-01), Gutterson et al.
patent: 2002/0157129 (2002-10-01), Perez et al.
patent: 92/04454 (1992-03-01), None
patent: 98/39462 (1998-09-01), None
patent: WO98/39462 (1998-09-01), None
patent: 00/37060 (2000-06-01), None
patent: 01/96583 (2001-12-01), None
Hanson et al 1999, The Plant Journal 19(6): 727-734.
Dotson et al., “A phosphonate monoester hydrolase fromBurkholderia caryophilliPG2982 is useful as a conditional lethal gene in plants”, Plant J. Aug. 1996;10(2):383-92.
Dotson et al., “Identification, characterization, and cloning of a phosphonate monoester hydrolase fromBurkholderia caryophilliPG2982”, J. Biol. Chem. Oct. 18, 1996;271(42):25754-61.
Ebinuma et al., “Selection of marker-free transgenic plants using the isopentenyl transferase gene”, Proc. Natl. Acad. Sci. USA Mar. 18, 1997;94(6):2117-2121.
Ellstrand et al., “Hybridization as an avenue of escape for engineered genes”, Bioscience Jun. 1990;40(6):438-442.
Gleave et al., “Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene”, Plant Mol. Biol. May 1999;40(2):223-35.
Hanson et al., “A simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences”, Plant J. Sep. 1999;19(6):727-34.
Huang et al., “Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors”, Transgenic Res. Oct. 2004;13(5):451-61.
Komari et al., “Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers”, Plant J. Jul. 1996;10(1):165-74.
Kononov et al., “Integration of T-DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration”, Plant J. May 1997;11(5):945-57.
Matthews et al., “Marker gene elimination from transgenic barley, using co-transformation with adjacent ‘twin TDNAs’ on a standard Agrobacterium transformation vector”, Mol. Breed. Sep. 7, 2001(3):195-202.
McCormac et al., “Efficient co-transformation ofNicotiana tabacumby two independent T-DNAs, the effect of T-DNA size and implications for genetic separation”, Transgenic Res. Apr. 2001;10(2):143-55.
Miller et al., “High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system”, Transgenic Res. Aug. 2002;11(4):381-96.
O'Keefe et al., “Plant Expression of a Bacterial Cytochrome P450 That Catalyzes Activation of a Sulfonylurea Pro-Herbicide”, Plant Physiol. Jun. 1994;105(2):473-482.
Patent Cooperation Treaty, International Search Report for PCT/US02/21350; May 8, 2003.
Rommens et al., “Crop improvement through modification of the plant's own genome”, Plant Physiol. May 2004;135(1):421-31.
Xing et al., “The use of the two T-DNA binary system to derive marker-free transgenic soybeans”, In Vitro Cell. Devel. Biol. Plant 2000; 36:456-463.
Yoder and Goldsbrough, “Transformation Systems for Generating Marker-Free Transgenic Plants”, Bio/Technology Mar.1994;12(3):263-267.
Armstrong Toni
Barton Kenneth
Crow Laura J.
Gilbertson Larry A.
Huang Shihshieh
Kruse David H
Monsanto Technology LLC
Thompson & Coburn LLP
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