Methods for enhancing segregation of transgenes in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C800S278000

Reexamination Certificate

active

07960610

ABSTRACT:
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

REFERENCES:
patent: 5627061 (1997-05-01), Barry et al.
patent: 5731179 (1998-03-01), Komari et al.
patent: 6521458 (2003-02-01), Gutterson et al.
patent: 2002/0157129 (2002-10-01), Perez et al.
patent: WO9204454 (1992-03-01), None
patent: WO9839462 (1998-09-01), None
patent: 99/01563 (1999-01-01), None
patent: WO0037060 (2000-06-01), None
patent: WO0196583 (2001-12-01), None
Lu et al Mar. 2001, Australian Journal of Plant Physiology vol. 28, pp. 241-248.
Dotson, Stanton, et al., A phosphonate monoester hydrolase fromBurkholderia caryophilliPG2982 is useful as a conditional lethal gene in plants, The Plant Journal, 1996, 383-392, vol. 10 No. 2.
Dotson, Stanton, et al., Identification, Characterization, and Cloning of a Phosphonate Monoester Hydrolase fromBurkholderia caryophilliPG2982, The Journal of Biological Chemistry, 1996, 25754-25761, vol. 271 No. 42.
Ebinuma, Hiroyasu, et al., Selection of marker-free transgenic plants using the isopentenyl transferase gene, Proceedings of the National Academy of Sciences USA, 1997, 2117-2121, vol. 94.
Ellstrand, Norman, et al., Hybridization as an Avenue of Escape for Engineered Genes, Bioscience, 1990, 438-442, vol. 40 No. 6.
Gleave, Andrew, et al., Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene, Plant Molecular Biology, 1999, 223-235, vol. 40.
Hanson, Bill, et al., A simple method to enrich anAgrobacterium-transformed population for plants containing only T-DNA sequences, The Plant Journal, 1999, 727-734, vol. 19 No. 6.
Huang, Shihshieh, et al., Generation of marker-free transgenic maize by regular two-borderAgrobacteriumtransformation vectors, Transgenic Research, 2004, 451-461, vol. 13.
Komari, Toshihiko, et al., Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated byAgrobacterium tumefaciensand segregation of transformants free from selection markers, The Plant Journal, 1996, 165-174, vol. 10 No. 1.
Kononov, Maria, et al., Integration of T-DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration, The Plant Journal, 1997, 945-957, vol. 11 No. 5.
Matthews, Peters, et al., Marker gene elimination from transgenic barley, using co-transformation with adjacent ‘twin T-DNAs’ on a standardAgrobacteriumtransformation vector, Molecular Breeding, 2001, 195-202, vol. 7.
McCormac, Alex, et al., Efficient co-transformation ofNicotiana tabacumby two independent T-DNAs, the effect of T-DNA size and implications for genetic separation, Transgenic Research, 2001, 143-155, vol. 10.
Miller, Michael, et al., High efficiency transgene segregation in co-transformed maize plants using anAgrobacterium tumefaciens2 T-DNA binary system, Transgenic Research, 2002, 381-392, vol. 11.
O'Keefe, Daniel, et al., Plant Expression of a Bacterial Cytochrome P450 That Catalyzes Activation of a Sulfonylurea Pro-Herbicide, Plant Physiology, 1994, 473-482, vol. 105.
International Search Report for PCT/US2002/021350 dated Feb. 4, 2003.
Rommens, Caius, et al., Crop Improvement through Modification of the Plant's Own Genome, Plant Physiology, 2004, 421-431, vol. 135.
Xing, Aiqiu, et al., The Use of the Two T-DNA Binary System to Derive Marker-Free Transgenic Soybeans, In Vitro Cellular and Developmental Biology, 2000, 456-463, vol. 36.
Yoder, John, et al., Transformation Systems for Generating Marker-Free Transgenic Plants, Bio/Technology, 1994, 263-267, vol. 12.
Martineau, Belinda et al., Letter to the Editor On Defining T-DNA, The Plant Cell, Aug. 1994, pp. 1032-1033, vol. 6.
Depicker, Ann et al., Frequencies of simultaneous transformation with different T-DNAs and their relevance to theAgrobacterium/plant cell interaction, Molecular Genetics and Genomics, 1985, pp. 477-484, vol. 201.
European Search Report for European Application No. 10171489.7-1212 dated Dec. 21, 2010, 7 pages.
Ramanathan, Vai. et al., Transfer of non-T-DNA portions of theAgrobacterium tumefaciensTi plasmid pTiA6 from the left terminus of TL-DNA, Plant Molecular Biology, 1995, pp. 1149-1154, vol. 28 No. 6.
Wang, Kan et al., Sequence context of the T-DNA border repeat element determines its relative activity during T-DNA transfer to plant cells, Molecular Genetics and Genomics, 1987, pp. 338-346, vol. 210.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for enhancing segregation of transgenes in plants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for enhancing segregation of transgenes in plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for enhancing segregation of transgenes in plants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2692124

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.