Methods for enhancing expression of recombinant proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

11358581

ABSTRACT:
The present invention provides two novelE. colisynthetic promoters, Syn 1 and Syn 2. Syn 1 and Syn 2 are two tight regulated synthetic promoters that control uninduced, leaky expression of proteins that are toxic and interfere with production of recombinant protein from anE. coliexpression system.

REFERENCES:
patent: 4551433 (1985-11-01), DeBoer
patent: 5545541 (1996-08-01), Molin et al.
patent: 2002/0085998 (2002-07-01), Jiang et al.
Amann et al.,Gene 25(2-3):167-178, 1983.
Boer et al.,Proc. Natl. Acad. Sci. 80(1):21-25, Jan. 1983.
Estrem et al.,Proc. Natl. Acad. Sci. USA 95(17):9761-9766, Aug. 1998.
Fernanadez et al.,Gene Expression Systems: Using Nature for the Art of Expression, Ch. 2, pp. 45-64, Academic Press, San Diego, 1999.
Ford et al.,Protein Expression and Purification 2(2-3):95-107, 1991.
Goldstein et al.,Biotechnol. Annu. Rev. 1:105-125, 1995.
Grussenmeyer et al.,Proc. Natl. Acad. Sci. USA 82(23):7952-7954, Dec. 1985.
Hopp et al.,Biotechnology 6:1204-1210, Oct. 1988.
Lee et al.,Cell 66(4):793-798, 1991.
Lisser et al.,Nucleic Acids Res. 21(7):1507-1516, 1993.
Mosrin-Huaman et al.,Mol. Mircobiol 51(5):1471-1481, 2004.
Nilsson et al.,EMBO J. 4(4):1075-1080, 1985.
Nilsson et al.,Methods Enzymol. 198:3-16, 1991.
Ross,J. Bacteriol. 180(20):5375-5383, Oct. 1998.
Smith et al.,Gene 67(1):31-40, 1988.
Straney et al.,Cell 51(5):699-707, Dec. 4, 1987.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for enhancing expression of recombinant proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for enhancing expression of recombinant proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for enhancing expression of recombinant proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3840438

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.