Methods for enhanced retrovirus-mediated gene transfer

Details Methods for enhanced retrovirus-mediated gene transfer Methods for enhanced retrovirus-mediated gene transfer

1994-03-25

1997-11-11

Guzo, David

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435372, C12N 1509, C12N 510

Patent

active

056862784

ABSTRACT:
A method to increase the efficiency of transduction of hematopoietic cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the hematopoietic cells, particularly including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on the enhanced gene transfer, and hematopoietic cellular populations.

REFERENCES:
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