Methods for eliminating mannosylphosphorylation of glycans...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

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C435S255100, C435S254200, C435S254230, C435S069100, C435S483000

Reexamination Certificate

active

11020808

ABSTRACT:
The present invention relates to the elimination of mannosylphosphorylation on the glycans of glycoproteins in the yeast genusPichia. The elimination of mannosylphosphorylated glycoproteins results from the disruption of the PNO1 gene and the newly isolatedP. pastorisMNN4B gene. The present invention further relates to methods for producing modified glycan structures in host cells that are free of glycan mannosylphosphorylation.

REFERENCES:
patent: 2002/0137134 (2002-09-01), Gerngross
patent: 1 283 265 (2001-05-01), None
patent: WO 01/88143 (2001-02-01), None
patent: WO 01/88143 (2001-11-01), None
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