Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-04-27
2009-10-20
Szperka, Michael (Department: 1644)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S004000, C435S007920, C424S438000, C424S811000, C514S002600
Reexamination Certificate
active
07604950
ABSTRACT:
Disclosed are methods for determining the pregnancy status of a cow by detecting a pregnancy-associated glycoprotein (PAG) level that comprises proteins present in an acidic protein fraction of day 55 to day 60 bovine placental tissue. These methods may involve using novel polyclonal and monoclonal antibodies that have been generated using the acidic protein fraction as an immunogen. According to the method, the cow is pregnant when the PAG level is present at an elevated level in a sample obtained from the cow. The invention provides accurate methods of detecting pregnancy at early stages and has the benefit of allowing early post partum detection of pregnancy status with few false positive results. The antibodies for detecting the PAG level will also be useful in combination with detecting elevated progesterone levels, thus providing an even more effective pregnancy detection method. The benefit of this early pregnancy detection is that identifying cows that are not pregnant very shortly after breeding allows for timely rebreeding and minimizes the amount of time the cow is open. Also disclosed are methods for making a breeding decision for a cow. These methods allow a herd manager to make breeding decisions based on the PAG level and optionally, progesterone levels, detected in a biological sample taken from a cow suspected of being in the early stages of pregnancy.
REFERENCES:
patent: 5455009 (1995-10-01), Vogler et al.
patent: 2003/0073248 (2003-04-01), Roth et al.
patent: 2007/0184558 (2007-08-01), Roth et al.
patent: WO 99/47934 (1999-09-01), None
patent: WO 03/043524 (2003-05-01), None
Gonzalez et al., Theriogenology, 1999, 52:717-725.
Ishiwata H et al. (2003), Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray,Molecular Reproduction and Development65:9-18.
Jonathan A. Green et al., “The Establishment of an ELISA for the Detection of Pregnancy-Associated Glycoproteins (PAGs) in the Serum of Pregnant Cows and Heifers”,Theriogenology, 63:1481-1503 (2005).
Osman V. Patel et al., “Quantitative Analysis Throughout Pregnancy of Placentomal and Interplacentomal Expression of Pregnancy-Associated Glycoproteins-1 and -9 in the Cow”,Molecular Reproduction and Development, 67:257-263 (2004).
Austin L. Hughes et al., “Adaptive Diversification Within a Large Family of Recently Duplicated, Placentally Expressed Genes”,PNAS, 97(7):3319-3323 (2000).
Jonathan A. Green et al., “Pregnancy-Associated Bovine and Ovine Glycoproteins Exhibit Spatially and Temporally Distinct Expression Patterns During Pregnancy”,Biology of Reproduction, 62:1624-1631 (2000).
Fan Huang et al., “Isolation, Purification, and Characterization of Pregnancy-Specific Protein B from Elk and Moose Placenta”,Biology of Reproduction, 61:1056-1061 (1999).
Jonathan A. Green et al., “Identification of a New Aspartic Proteinase Expressed by the Outer Chorionic Cell Layer of the Equine Placenta”,Biology of Reproduction, 60:1069-1077 (1999).
O. Szenci et al., “Comparison of Ultrasonography, Bovine Pregnancy-Specific Protein B, and Bovine Pregnancy-Associated Glycoprotein 1 Tests for Pregnancy Detection in Dairy Cows”,Theriogenology50:77-88 (1998).
Sancai Xie et al., “The Diversity and Evolutionary Relationships of the Pregnancy-Associated Glycoproteins, an Aspartic Proteinase Subfamily Consisting of Many Trophoblast-Expressed Genes”,Proc. Natl. Acad. Sci., 94:12809-12816 (1997).
Nagappan Mathialagan et al., “Pepsin-Inhibitory Activity of the Uterine Serpins,”Proc. Natl. Acad. Sci., 93:13653-13658 (1996).
G.H. Kiracofe et al., “Pregnancy-Specific Protein B in Serum of Postpartum Beef Cows”,J. Anim. Sci., 71:2199-2205 (1993).
Sancai Xie et al., “Identification of the Major Pregnancy-Specific Antigens of Cattle and Sheep as Inactive Members of the Aspartic Proteinase Family”,Proc. Natl. Acad Sci., 88:10247-10251 (1991).
Andre Pagnah Zoli et al., “Purification and Characterization of a Bovine Pregnancy-Associated Glycoprotein”,Biology of Reproduction, 45:1-10 (1991).
Mathialagan Nagappan
McGrath Michael
Schenkel Robert
Howrey LLP
Madsen Matthew L.
Monsanto Technology LLC
Szperka Michael
LandOfFree
Methods for early detection of pregnancy in cows does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for early detection of pregnancy in cows, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for early detection of pregnancy in cows will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4139042