Methods for DNA amplification and sequencing

Details Methods for DNA amplification and sequencing Methods for DNA amplification and sequencing

2006-12-19

2006-12-19

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091200

Reexamination Certificate

active

07150980

ABSTRACT:
The osmoprotectants proline, 2-methyl-4-carboxy-3,4,5,6-tetrahydropyrimidine (“THP(B)”, and 2-methyl-4-carboxy-5-hydroxy-3,4,5,6,-tetrahydropyrimidine (“THP(A)”) are capable of increasing the thermal stability of DNA polymerases at elevated temperatures. THP(B) is further effective in lowering the melting temperature of double-stranded DNA. Proline, THP(A) and THP(B) are thus useful in procedures involving melting of double-stranded DNA and/or polymerase-mediated DNA synthesis, such as in primer extension, in PCR (polymerase chain reaction) amplification and in DNA sequencing.

REFERENCES:
patent: 6268133 (2001-07-01), Nisson et al.
patent: 6875568 (2005-04-01), Nisson et al.
patent: 0 553 884 (1993-08-01), None
patent: 94 23055 (1994-10-01), None
patent: 95 20682 (1995-08-01), None
patent: 96 12041 (1996-04-01), None
patent: 96 34115 (1996-10-01), None
patent: 97 20949 (1997-06-01), None
Thakar et al., “Osmolyte mediation of T7 DNA polymerase and plasmid DNA stability”, BIOCHEMISTRY, vol. 33(44):12255-12259, Oct. 11, 1994.
Iakobashvili et al., “Low temperature cycled PCR protocol fr klenow fragement of DNA polymerase I in the presence of proline”, Nucleic Acids Research, vol. 27 (6)1566-8, Mar. 1999.
Rajendrakumar et al., “DNA helix destabilization by proline and betaine: possible role in the salinity tolerance stress”, FEBS Letters, vol. 410:210-205 (1997).

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