Methods for discouraging bacterial growth in culture medium

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S257100, C426S417000, C426S601000, C426S648000

Reexamination Certificate

active

08008050

ABSTRACT:
Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellateCrypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

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