Methods for direct visualization of active synapses

Details Methods for direct visualization of active synapses Methods for direct visualization of active synapses

2011-04-12

2011-04-12

Chen, Shin-Lin (Department: 1632)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Fusion protein or fusion polypeptide

C435S007720, C435S320100, C435S455000, C530S350000, C536S023400

Reexamination Certificate

active

07923015

ABSTRACT:
A method for visualizing an active synapse wherein said method comprises: (a) exposing cells forming the active synapse to a biomarker comprising at least fragment C of tetanus toxin and a reporter protein; and (b) visualizing the biomarker; wherein the accumulation of the biomarker into dendritic spines of the cells allows visualization of an active synapse. Also, a method for screening molecules capable of modulating synapse activity is provided. A kit useful for the early diagnosis of neurodegenerative disease comprises a biomarker comprising at least fragment C of tetanus toxin and a reporter protein.

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