Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2011-08-30
2011-08-30
Cheu, Jacob (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007910, C435S007920, C436S518000
Reexamination Certificate
active
08008026
ABSTRACT:
The present invention relates to a method for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide in a biosample, which comprises the steps of: (a) preparing a carrier-capturing antibody conjugate by binding a capturing antibody to the surface of a solid phase carrier in a three dimensional manner, wherein the capturing antibody is capable of recognizing an epitope on the multimer-forming polypeptide; (b) preparing a detection antibody, wherein an epitope recognized by the detection antibody is present at a position in the multimer-forming polypeptide to cause a steric hindrance by the capturing antibody bound to its epitope to prevent the binding of the detection antibody to the multimer-forming polypeptide; (c) contacting simultaneously the carrier-capturing antibody conjugate and the detection antibody to the biosample; and (d) detecting the formation of a carrier-capturing antibody-multimeric form-detection antibody complex.
REFERENCES:
patent: 4208479 (1980-06-01), Zuk et al.
patent: 6765088 (2004-07-01), Korth et al.
patent: 6846640 (2005-01-01), Peach et al.
patent: 6913896 (2005-07-01), Raven et al.
patent: 2006/0057671 (2006-03-01), Orser et al.
patent: 2007/0077603 (2007-04-01), Heeb et al.
patent: 02/097444 (2002-12-01), None
patent: 2006/088281 (2008-10-01), None
Serbec et al. J Biol. Chem. 2004 vol. 279, p. 3694-3698.
International Search Report (ISR) dated Aug. 1, 2007, for International Patent Application PCT/KR2007/001947.
Jiri Safar, et al., “Eight prion strains have PrPSc molecules with different conformations”, Nature Medicine, Oct. 1998, pp. 1157-1165, vol. 4, No. 10.
An Seong Soo Alexander
Lim Kun Taek
Oh Hyun Jung
Cheu Jacob
Goldberg Joshua B.
Koh Mihsuhn
Peoplebio, Inc.
The Nath Law Group
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